RT Journal Article
T1 First isolation of Clostridioides difficile from smoked and dried freshwater fish in Cambodia
A1 Rodriguez, Cristina
A1 Mith, Hasika
A1 Taminiau, Bernard
A1 Bouchafa, Lamia
A1 Van Broeck, Johan
A1 Soumillion, Kate
A1 Ngyuvula, Eleonore
A1 Garcia-Fuentes, Eduardo
A1 Korsak, Nicolas
A1 Delmee, Michel
A1 Daube, Georges
K1 Clostridioides difficile
K1 Smoked dried freshwater fish
K1 Cross-contamination
K1 Ready-to-eat food
K1 Antibiotic resistance
K1 Food contamination
K1 Retail meat
K1 Antibiotic-resistance
K1 Susceptibility
K1 Contamination
K1 Vegetables
K1 Food
K1 Environment
K1 Prevalence
K1 Seafood
K1 Humans
AB In Cambodia, freshwater aquaculture is the most important source of food production. Fresh fish meat is considered a highly perishable food that requires the use of different manipulations and preservation techniques to inhibit the proliferation of undesirable bacteria. These bacteria are naturally present in the raw product or could be acquired during manipulation by cross-contamination. Many studies worldwide have investigated the epidemiology of Clostridioides difficile (C. difficile) in food, but to date, there are no publications about the bacterium in ready-to-eat fish or descriptions in Cambodia. The objective of this study was to assess the presence of C. difficile in one of the main food supplies of this country, smoked freshwater fish, originating from different provinces. A total of 25 samples were collected directly from local markets, yielding 4 C. difficile isolates and an overall recovery rate of 16%. Most of the isolates were toxigenic and classified as rare PCR profiles, and they were resistant to clindamycin. These findings indicate contamination during handling and/or contamination of the raw fish, followed by insufficient heat treatment to kill the spores. The presence of C. difficile in smoked and dried fish implies a potential risk of human exposure, contamination and infection.
PB Elsevier sci ltd
SN 0956-7135
YR 2021
FD 2021-02-10
LK http://hdl.handle.net/10668/18778
UL http://hdl.handle.net/10668/18778
LA en
DS RISalud
RD Apr 10, 2025