RT Journal Article
T1 Neuronal Metabolism and Neuroprotection: Neuroprotective Effect of Fingolimod on Menadione-Induced Mitochondrial Damage
A1 Gil, Antonio
A1 Martín-Montañez, Elisa
A1 Valverde, Nadia
A1 Lara, Estrella
A1 Boraldi, Federica
A1 Claros, Silvia
A1 Romero-Zerbo, Silvana-Yanina
A1 Fernández, Oscar
A1 Pavia, Jose
A1 Garcia-Fernandez, Maria
K1 Sphingosine-1-phosphate receptor analogue
K1 Fingolimod phosphate
K1 Neuroprotection
K1 Mitochondrial damage
K1 Glycolytic pathway
K1 Pentose phosphate pathway
K1 Redox homeostasis
K1 Receptores de esfingosina-1-fosfato
K1 Neuroprotección
K1 Mitocondrias
K1 Glucólisis
K1 Vía de la pentosa-fosfato
K1 Oxidación-reducción
K1 Homeostasis
AB Imbalance in the oxidative status in neurons, along with mitochondrial damage, are common characteristics in some neurodegenerative diseases. The maintenance in energy production is crucial to face and recover from oxidative damage, and the preservation of different sources of energy production is essential to preserve neuronal function. Fingolimod phosphate is a drug with neuroprotective and antioxidant actions, used in the treatment of multiple sclerosis. This work was performed in a model of oxidative damage on neuronal cell cultures exposed to menadione in the presence or absence of fingolimod phosphate. We studied the mitochondrial function, antioxidant enzymes, protein nitrosylation, and several pathways related with glucose metabolism and glycolytic and pentose phosphate in neuronal cells cultures. Our results showed that menadione produces a decrease in mitochondrial function, an imbalance in antioxidant enzymes, and an increase in nitrosylated proteins with a decrease in glycolysis and glucose-6-phosphate dehydrogenase. All these effects were counteracted when fingolimod phosphate was present in the incubation media. These effects were mediated, at least in part, by the interaction of this drug with its specific S1P receptors. These actions would make this drug a potential tool in the treatment of neurodegenerative processes, either to slow progression or alleviate symptoms.
PB MDPI
YR 2020
FD 2020-12-29
LK http://hdl.handle.net/10668/4508
UL http://hdl.handle.net/10668/4508
LA en
NO Gil A, Martín-Montañez E, Valverde N, Lara E, Boraldi F, Claros S, et al. Neuronal Metabolism and Neuroprotection: Neuroprotective Effect of Fingolimod on Menadione-Induced Mitochondrial Damage. Cells. 2020 Dec 29;10(1):34
DS RISalud
RD Apr 12, 2025