RT Journal Article
T1 Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells.
A1 Sabri, Farideh
A1 Prados, Alejandro
A1 Muñoz-Fernández, Raquel
A1 Lantto, Rebecka
A1 Fernandez-Rubio, Pablo
A1 Nasi, Aikaterini
A1 Amu, Sylvie
A1 Albert, Jan
A1 Garcia Olivares, Enrique
A1 Chiodi, Francesca
K1 Follicular dendritic cells
K1 HIV-1
K1 Chemokines
K1 Inflammation
K1 B cells
K1 Presentación de antígeno
K1 Linfocitos B
K1 Línea celular
K1 Supervivencia celular
K1 Citocinas
K1 Células dendríticas foliculares
K1 Genoma viral
K1 Centro germinal
K1 VIH-1
K1 Tejido linfoide
K1 Receptores del VIH
AB BACKGROUNDFollicular dendritic cells (FDCs) are important components in the organization of germinal centers in lymphoid tissue where, following antigen presentation, B cells differentiate into memory B cells. The possibility of establishing primary cell lines from FDCs isolated from lymphoid tissue paved the way for characterization of FDC biological properties. We exposed primary FDC cell lines to HIV-1 strains in vitro and studied changes in the chemo-attractive properties of FDCs and release of inflammatory cytokines.RESULTSFDC lines expressed several known and putative HIV-1 receptors; viral genome was amplified in HIV-1 exposed FDCs which released low levels of p24 HIV-1 protein in culture supernatants, but were not definitely proven to be productively infected. Exposure of FDCs to HIV-1 strains did not change the expression of markers used to characterize these cells. HIV-1 exposed FDCs, however, changed the expression of chemo-attractants involved in cell recruitment at inflammatory sites and increased the production of several inflammatory cytokines. The inflammatory milieu created upon HIV-1 exposure of FDCs led to impaired B cell survival in vitro and reduced Ig production.CONCLUSIONSFDC lines exposed to different HIV-1 strains, although not able to support productive HIV-1 replication, show an increased production of inflammatory cytokines. Our in vitro model of interactions between HIV-1 exposed FDC lines and B cells suggest that exposure of FDCs to HIV-1 in vivo can contribute to inflammation within germinal centers and that this pathological event may impair B cell survival and contribute to impaired B cell responses during HIV-1 infection.
PB BioMed Central
YR 2016
FD 2016-09-05
LK http://hdl.handle.net/10668/2517
UL http://hdl.handle.net/10668/2517
LA en
NO Sabri F, Prados A, Muñoz-Fernández R, Lantto R, Fernandez-Rubio P, Nasi A, et al. Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells. Retrovirology. 2016; 13(1):61
NO Journal Article;
DS RISalud
RD Apr 12, 2025