%0 Journal Article
%A Sabri, Farideh
%A Prados, Alejandro
%A Muñoz-Fernández, Raquel
%A Lantto, Rebecka
%A Fernandez-Rubio, Pablo
%A Nasi, Aikaterini
%A Amu, Sylvie
%A Albert, Jan
%A Garcia Olivares, Enrique
%A Chiodi, Francesca
%T Impaired B cells survival upon production of inflammatory cytokines by HIV-1 exposed follicular dendritic cells.
%D 2016
%U http://hdl.handle.net/10668/2517
%X BACKGROUNDFollicular dendritic cells (FDCs) are important components in the organization of germinal centers in lymphoid tissue where, following antigen presentation, B cells differentiate into memory B cells. The possibility of establishing primary cell lines from FDCs isolated from lymphoid tissue paved the way for characterization of FDC biological properties. We exposed primary FDC cell lines to HIV-1 strains in vitro and studied changes in the chemo-attractive properties of FDCs and release of inflammatory cytokines.RESULTSFDC lines expressed several known and putative HIV-1 receptors; viral genome was amplified in HIV-1 exposed FDCs which released low levels of p24 HIV-1 protein in culture supernatants, but were not definitely proven to be productively infected. Exposure of FDCs to HIV-1 strains did not change the expression of markers used to characterize these cells. HIV-1 exposed FDCs, however, changed the expression of chemo-attractants involved in cell recruitment at inflammatory sites and increased the production of several inflammatory cytokines. The inflammatory milieu created upon HIV-1 exposure of FDCs led to impaired B cell survival in vitro and reduced Ig production.CONCLUSIONSFDC lines exposed to different HIV-1 strains, although not able to support productive HIV-1 replication, show an increased production of inflammatory cytokines. Our in vitro model of interactions between HIV-1 exposed FDC lines and B cells suggest that exposure of FDCs to HIV-1 in vivo can contribute to inflammation within germinal centers and that this pathological event may impair B cell survival and contribute to impaired B cell responses during HIV-1 infection.
%K Follicular dendritic cells
%K HIV-1
%K Chemokines
%K Inflammation
%K B cells
%K Presentación de antígeno
%K Linfocitos B
%K Línea celular
%K Supervivencia celular
%K Citocinas
%K Células dendríticas foliculares
%K Genoma viral
%K Centro germinal
%K VIH-1
%K Tejido linfoide
%K Receptores del VIH
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