RT Journal Article
T1 Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza.
A1 Bermejo-Martin, Jesus F
A1 Ortiz de Lejarazu, Raul
A1 Pumarola, Tomas
A1 Rello, Jordi
A1 Almansa, Raquel
A1 Ramírez, Paula
A1 Martin-Loeches, Ignacio
A1 Varillas, David
A1 Gallegos, Maria C
A1 Serón, Carlos
A1 Micheloud, Dariela
A1 Gomez, Jose Manuel
A1 Tenorio-Abreu, Alberto
A1 Ramos, María J
A1 Molina, M Lourdes
A1 Huidobro, Samantha
A1 Sanchez, Elia
A1 Gordón, Mónica
A1 Fernández, Victoria
A1 Del Castillo, Alberto
A1 Marcos, Ma Angeles
A1 Villanueva, Beatriz
A1 López, Carlos Javier
A1 Rodríguez-Domínguez, Mario
A1 Galan, Juan-Carlos
A1 Cantón, Rafael
A1 Lietor, Aurora
A1 Rojo, Silvia
A1 Eiros, Jose M
A1 Hinojosa, Carmen
A1 Gonzalez, Isabel
A1 Torner, Nuria
A1 Banner, David
A1 Leon, Alberto
A1 Cuesta, Pablo
A1 Rowe, Thomas
A1 Kelvin, David J
K1 Quimiocinas
K1 Citocinas
K1 Cartilla de ADN
K1 Femenino
K1 Pruebas de Inhibición de Hemaglutinación
K1 Humanos
K1 Subtipo H1N1 del Virus de la Influenza A
K1 Gripe Humana
K1 Unidades de Cuidados Intensivos
K1 Tiempo de Internación
K1 Masculino
K1 Mediana Edad
K1 Selección de Paciente
K1 ARN Viral
K1 Índice de Severidad de la Enfermedad
K1 Células TH1
K1 Carga Viral
K1 Adulto
AB INTRODUCTIONHuman host immune response following infection with the new variant of A/H1N1 pandemic influenza virus (nvH1N1) is poorly understood. We utilize here systemic cytokine and antibody levels in evaluating differences in early immune response in both mild and severe patients infected with nvH1N1.METHODSWe profiled 29 cytokines and chemokines and evaluated the haemagglutination inhibition activity as quantitative and qualitative measurements of host immune responses in serum obtained during the first five days after symptoms onset, in two cohorts of nvH1N1 infected patients. Severe patients required hospitalization (n = 20), due to respiratory insufficiency (10 of them were admitted to the intensive care unit), while mild patients had exclusively flu-like symptoms (n = 15). A group of healthy donors was included as control (n = 15). Differences in levels of mediators between groups were assessed by using the non parametric U-Mann Whitney test. Association between variables was determined by calculating the Spearman correlation coefficient. Viral load was performed in serum by using real-time PCR targeting the neuraminidase gene.RESULTSIncreased levels of innate-immunity mediators (IP-10, MCP-1, MIP-1beta), and the absence of anti-nvH1N1 antibodies, characterized the early response to nvH1N1 infection in both hospitalized and mild patients. High systemic levels of type-II interferon (IFN-gamma) and also of a group of mediators involved in the development of T-helper 17 (IL-8, IL-9, IL-17, IL-6) and T-helper 1 (TNF-alpha, IL-15, IL-12p70) responses were exclusively found in hospitalized patients. IL-15, IL-12p70, IL-6 constituted a hallmark of critical illness in our study. A significant inverse association was found between IL-6, IL-8 and PaO2 in critical patients.CONCLUSIONSWhile infection with the nvH1N1 induces a typical innate response in both mild and severe patients, severe disease with respiratory involvement is characterized by early secretion of Th17 and Th1 cytokines usually associated with cell mediated immunity but also commonly linked to the pathogenesis of autoimmune/inflammatory diseases. The exact role of Th1 and Th17 mediators in the evolution of nvH1N1 mild and severe disease merits further investigation as to the detrimental or beneficial role these cytokines play in severe illness.
PB BIOMED CENTRAL LTD
SN 1364-8535
YR 2009
FD 2009-12-11
LK http://hdl.handle.net/10668/1670
UL http://hdl.handle.net/10668/1670
LA en
NO Bermejo-Martin JF, Ortiz de Lejarazu R, Pumarola T, Rello J, Almansa R, Ramírez P, et al. Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza. Crit Care. 2009; 13(6):R201
NO Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 6, 2025