%0 Journal Article
%A Rodriguez-Temporal, David
%A Alcaide, Fernando
%A Mareković, Ivana
%A O'Connor, James Anthony
%A Gorton, Rebecca
%A van Ingen, Jakko
%A Van den Bossche, An
%A Héry-Arnaud, Genevieve
%A Beauruelle, Clémence
%A Orth-Höller, Dorothea
%A Palacios-Gutiérrez, Juan-José
%A Tudó, Griselda
%A Bou, Germán
%A Ceyssens, Pieter-Jan
%A Garrigó, Montserrat
%A González-Martin, Julià
%A Greub, Gilbert
%A Hrabak, Jaroslav
%A Ingebretsen, André
%A Mediavilla-Gradolph, Maria Concepción
%A Oviaño, Marina
%A Palop, Begoña
%A Pranada, Arthur B
%A Quiroga, Lidia
%A Ruiz-Serrano, Maria Jesús
%A Rodríguez-Sánchez, Belén
%T Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification.
%D 2022
%U http://hdl.handle.net/10668/19582
%X The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
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