RT Journal Article
T1 Hydroxyl Groups Induce Bioactivity in Silica/Chitosan Aerogels Designed for Bone Tissue Engineering. In Vitro Model for the Assessment of Osteoblasts Behavior
A1 Perez-Moreno, Antonio
A1 Reyes-Peces, María de las Virtudes
A1 de Los Santos, Deseada María
A1 Pinaglia-Tobaruela, Gonzalo
A1 de la Orden, Emilio
A1 Vilches-Pérez, José Ignacio
A1 Salido, Mercedes
A1 Piñero, Manuel
A1 de la Rosa-Fox, Nicolás
K1 Aerogels
K1 Biomaterials
K1 Fracture toughness
K1 Hydroxyapatite (HAp)
K1 Bone tissue engineering
K1 Osteoinduction
K1 Osteoblasts
K1 Chitosan
K1 Focal adhesions
K1 Materiales biocompatibles
K1 Osteoblastos
K1 Quitosano
AB Silica (SiO2)/chitosan (CS) composite aerogels are bioactive when they are submerged in simulated body fluid (SBF), causing the formation of bone-like hydroxyapatite (HAp) layer. Silica-based hybrid aerogels improve the elastic behavior, and the combined CS modifies the network entanglement as a crosslinking biopolymer. Tetraethoxysilane (TEOS)/CS is used as network precursors by employing a sol-gel method assisted with high power ultrasound (600 W). Upon gelation and aging, gels are dried in supercritical CO2 to obtain monoliths. Thermograms provide information about the condensation of the remaining hydroxyl groups (400-700 °C). This step permits the evaluation of the hydroxyl group's content of 2 to 5 OH nm-2. The formed Si-OH groups act as the inductor of apatite crystal nucleation in SBF. The N2 physisorption isotherms show a hysteresis loop of type H3, characteristic to good interconnected porosity, which facilitates both the bioactivity and the adhesion of osteoblasts cells. After two weeks of immersion in SBF, a layer of HAp microcrystals develops on the surface with a stoichiometric Ca/P molar ratio of 1.67 with spherulite morphology and uniform sizes of 6 μm. This fact asserts the bioactive behavior of these hybrid aerogels. Osteoblasts are cultured on the selected samples and immunolabeled for cytoskeletal and focal adhesion expression related to scaffold nanostructure and composition. The initial osteoconductive response observes points to a great potential of tissue engineering for the designed composite aerogels.
PB MDPI
YR 2020
FD 2020-11-26
LK http://hdl.handle.net/10668/4255
UL http://hdl.handle.net/10668/4255
LA en
NO Perez-Moreno A, Reyes-Peces MV, de Los Santos DM, Pinaglia-Tobaruela G, de la Orden E, Vilches-Pérez JI, et al. Hydroxyl Groups Induce Bioactivity in Silica/Chitosan Aerogels Designed for Bone Tissue Engineering. In Vitro Model for the Assessment of Osteoblasts Behavior. Polymers. 2020 Nov 26;12(12):2802
DS RISalud
RD Apr 10, 2025