RT Journal Article
T1 Deciphering the Resistome of the Widespread Pseudomonas aeruginosa Sequence Type 175 International High-Risk Clone through Whole-Genome Sequencing
A1 Cabot, Gabriel
A1 Lopez-Causape, Carla
A1 Ocampo-Sosa, Alain A.
A1 Sommer, Lea M.
A1 Angeles Dominguez, Maria
A1 Zamorano, Laura
A1 Juan, Carlos
A1 Tubau, Fe
A1 Rodriguez, Cristina
A1 Moya, Bartolome
A1 Pena, Carmen
A1 Martinez-Martinez, Luis
A1 Plesiat, Patrick
A1 Oliver, Antonio
A1 Spanish Network Res Infect Dis, 
K1 Beta-lactam resistance
K1 Penicillin-binding proteins
K1 Blood-stream infections
K1 Antibiotic-resistance
K1 Antimicrobial resistance
K1 Clinical strains
K1 St175 clone
K1 Virulence
K1 Oprm
K1 Mechanisms
AB Whole-genome sequencing (WGS) was used for the characterization of the frequently extensively drug resistant (XDR) Pseudomonas aeruginosa sequence type 175 (ST175) high-risk clone. A total of 18 ST175 isolates recovered from 8 different Spanish hospitals were analyzed; 4 isolates from 4 different French hospitals were included for comparison. The typical resistance profile of ST175 included penicillins, cephalosporins, monobactams, carbapenems, aminoglycosides, and fluoroquinolones. In the phylogenetic analysis, the four French isolates clustered together with two isolates from one of the Spanish regions. Sequence variation was analyzed for 146 chromosomal genes related to antimicrobial resistance, and horizontally acquired genes were explored using online databases. The resistome of ST175 was determined mainly by mutational events; resistance traits common to all or nearly all of the strains included specific ampR mutations leading to ampC overexpression, specific mutations in oprD conferring carbapenem resistance, or a mexZ mutation leading to MexXY overexpression. All isolates additionally harbored an aadB gene conferring gentamicin and tobramycin resistance. Several other resistance traits were specific to certain geographic areas, such as a streptomycin resistance gene, aadA13, detected in all four isolates from France and in the two isolates from the Cantabria region and a glpT mutation conferring fosfomycin resistance, detected in all but these six isolates. Finally, several unique resistance mutations were detected in single isolates; particularly interesting were those in genes encoding penicillin-binding proteins (PBP1A, PBP3, and PBP4). Thus, these results provide information valuable for understanding the genetic basis of resistance and the dynamics of the dissemination and evolution of high-risk clones.
PB Amer soc microbiology
SN 0066-4804
YR 2016
FD 2016-12-01
LK https://hdl.handle.net/10668/27412
UL https://hdl.handle.net/10668/27412
LA en
DS RISalud
RD Apr 17, 2025