%0 Journal Article
%A Perez, Cristina
%A Botta, Cirino
%A Zabaleta, Aintzane
%A Puig, Noemi
%A Cedena, Maria-Teresa
%A Goicoechea, Ibai
%A Alameda, Daniel
%A San José-Eneriz, Edurne
%A Merino, Juana
%A Rodríguez-Otero, Paula
%A Maia, Catarina
%A Alignani, Diego
%A Maiso, Patricia
%A Manrique, Irene
%A Lara-Astiaso, David
%A Vilas-Zornoza, Amaia
%A Sarvide, Sarai
%A Riillo, Caterina
%A Rossi, Marco
%A Rosiñol, Laura
%A Oriol, Albert
%A Blanchard, María-Jesús
%A Rios, Rafael
%A Sureda, Anna
%A Martin, Jesus
%A Martinez, Rafael
%A Bargay, Joan
%A de la Rubia, Javier
%A Hernandez, Miguel-Teodoro
%A Martinez-Lopez, Joaquin
%A Orfao, Alberto
%A Agirre, Xabier
%A Prosper, Felipe
%A Mateos, Maria-Victoria
%A Lahuerta, Juan-José
%A Blade, Joan
%A San-Miguel, Jesús F
%A Paiva, Bruno
%T Immunogenomic identification and characterization of granulocytic myeloid-derived suppressor cells in multiple myeloma.
%D 2020
%U http://hdl.handle.net/10668/15418
%X Granulocytic myeloid-derived suppressor cells (G-MDSCs) promote tumor growth and immunosuppression in multiple myeloma (MM). However, their phenotype is not well established for accurate monitoring or clinical translation. We aimed to provide the phenotypic profile of G-MDSCs based on their prognostic significance in MM, immunosuppressive potential, and molecular program. The preestablished phenotype of G-MDSCs was evaluated in bone marrow samples from controls and MM patients using multidimensional flow cytometry; surprisingly, we found that CD11b+CD14-CD15+CD33+HLADR- cells overlapped with common eosinophils and neutrophils, which were not expanded in MM patients. Therefore, we relied on automated clustering to unbiasedly identify all granulocytic subsets in the tumor microenvironment: basophils, eosinophils, and immature, intermediate, and mature neutrophils. In a series of 267 newly diagnosed MM patients (GEM2012MENOS65 trial), only the frequency of mature neutrophils at diagnosis was significantly associated with patient outcome, and a high mature neutrophil/T-cell ratio resulted in inferior progression-free survival (P
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