RT Journal Article
T1 New evidence for dietary fatty acids in the neutrophil traffic between the bone marrow and the peripheral blood.
A1 Ortega-Gomez, Almudena
A1 Lopez, Sergio
A1 Varela, Lourdes M
A1 Jaramillo, Sara
A1 Muriana, Francisco J G
A1 Abia, Rocio
K1 BMSF, bone marrow supernatant fluid
K1 Bone marrow inflammation
K1 Butter
K1 Ct, threshold cycle
K1 DHA, docosahexaenoic acid
K1 Dietary fatty acids
K1 EPA, eicosapentaenoic acid
K1 FACS, fluorescence-activated cell sorting
K1 FSC, forward scatter
K1 HBSS, Hank’s balance salt solution
K1 HFDs, high-fat diets
K1 HSCs, hematopoietic stem cells
K1 High-fat diets
K1 LFD, low-fat diet
K1 MFI, mean fluorescence intensity
K1 MMP9, matrix metalloproteinase 9
K1 MUFAs, monounsaturated fatty acids
K1 Neutrophil mobilisation
K1 OCM, oral control meal
K1 OFLs, oral fat loads
K1 OFMs, oral fat meals
K1 OSL, oral saline load
K1 Olive oil
K1 PI, propidium iodide
K1 PUFAs, polyunsaturated fatty acids
K1 SFAs, saturated fatty acids
K1 SSC, side scatter
K1 TRLs, triglyceride-rich lipoproteins
K1 qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction
AB Chronic administration of a high-fat diet in mice has been established to influence the generation and trafficking of immune cells such as neutrophils in the bone marrow, the dysregulation of which may contribute to a wide range of diseases. However, no studies have tested the hypothesis that a short-term, high-fat diet could early modulate the neutrophil release from bone marrow at fasting and at postprandial in response to a high-fat meal challenge, and that the predominant type of fatty acids in dietary fats could play a role in both context conditions. Based on these premises, we aimed to establish the effects of different fats [butter, enriched in saturated fatty acids (SFAs), olive oil, enriched in monounsaturated fatty acids (MUFAs), and olive oil supplemented with eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids] on neutrophil navigation from bone marrow to blood in mice. The analysis of cellular models for mechanistic understanding and of postprandial blood samples from healthy volunteers for translational purposes was assessed. The results revealed a powerful effect of dietary SFAs in promotion the neutrophil traffic from bone marrow to blood via the CXCL2-CXCR2 axis. Dietary SFAs, but not MUFAs or EPA and DHA, were also associated with increased neutrophil apoptosis and bone marrow inflammation. Similar dietary fatty-acid-induced postprandial neutrophilia was observed in otherwise healthy humans. Therefore, dietary MUFAs might preserve bone marrow health and proper migration of bone marrow neutrophils early in the course of high-fat diets even after the intake of high-fat meals.
YR 2022
FD 2022-09-06
LK http://hdl.handle.net/10668/22231
UL http://hdl.handle.net/10668/22231
LA en
DS RISalud
RD Apr 19, 2025