%0 Journal Article
%A Mascaraque, Ainhoa
%A Kowalczyk, Wioleta
%A Fernández, Tahia
%A Palomares, Francisca
%A Mayorga, Cristobalina
%A Andreu, David
%A Rojo, Javier
%T Glycodendropeptides stimulate dendritic cell maturation and T cell proliferation: a potential influenza A virus immunotherapy
%D 2015
%@ 2040-2511
%U http://hdl.handle.net/10668/2274
%X Mannosylation facilitates uptake and internalization of immunogenic peptides by antigen-processing cells expressing mannose receptors at their surface, such as DC-SIGN, a lectin that plays a key role in the immune response against different pathogens. This internalization, processing and subsequent MHC presentation may result in a strong T cell stimulation. Here, we hypothesized that combining mannose glycodendrons with multivalent presentation of peptide epitopes in a likewise dendron format would yieldhybrid constructs, named glycodendropeptides (GDPs), with the capacity to enhance peptide immunogenicity, hence providing a novel and versatile platform for applications in immunotherapy. Thus, GDPs of differentvalencies displaying the NP366–374 epitope, a conserved sequence from the influenza A virus nucleoprotein (NP), have been built by two click chemistry-based methodologies and assessed as potential flu vaccine candidates. Preliminary evaluation of the ability of these constructs to stimulate dendritic cell maturation and lymphocyte proliferation was promising, showing the highest-functionalized NP366–374 GDPs as inducing the strongest immunostimulatory effect.
%K Moléculas de adhesión celular
%K Química clic
%K Secuencia conservada
%K Dendrímeros
%K Células dendríticas
%K Epítopos
%K Inmunoterapia
%K Lectinas tipo C
%K Manosa
%K Lectinas de unión a manosa
%K Péptidos
%K Proteínas de unión al ARN
%K Receptores de superficie celular
%K Linfocitos T
%K Vacunas
%K Proteínas del centro vírico
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