RT Journal Article
T1 Gut Microbiota Has a Crucial Role in the Development of Hypertension and Vascular Dysfunction in Toll-like Receptor 7-Driven Lupus Autoimmunity.
A1 de la Visitación, Néstor
A1 Robles-Vera, Iñaki
A1 Moleón, Javier
A1 González-Correa, Cristina
A1 Aguilera-Sánchez, Nazaret
A1 Toral, Marta
A1 Gómez-Guzmán, Manuel
A1 Sánchez, Manuel
A1 Jiménez, Rosario
A1 Martin-Morales, Natividad
A1 O'Valle, Francisco
A1 Romero, Miguel
A1 Duarte, Juan
K1 TLR7 activation
K1 endothelial dysfunction
K1 gut dysbiosis
K1 hypertension
K1 immune system
K1 systemic lupus erythematosus
AB Our group has investigated the involvement of gut microbiota in hypertension in a murine model of systemic lupus erythematosus induced by Toll-like receptor (TLR)-7 activation. Female BALB/c mice were randomly assigned to four experimental groups: an untreated control (CTR), a group treated with the TLR7 agonist imiquimod (IMQ), IMQ-treated with vancomycin, and IMQ-treated with a cocktail of broad-spectrum antibiotics. We carried out faecal microbiota transplant (FMT) from donor CTR or IMQ mice to recipient IMQ or CTR animals, respectively. Vancomycin inhibited the increase in blood pressure; improved kidney injury, endothelial function, and oxidative stress; and reduced T helper (Th)17 infiltration in aortas from IMQ-treated mice. The rise in blood pressure and vascular complications present in IMQ mice were also observed in the CTR mice recipients of IMQ microbiota. Reduced relative populations of Sutterella and Anaerovibrio were associated with high blood pressure in our animals, which were increased after stool transplantation of healthy microbiota to IMQ mice. The reduced endothelium-dependent vasodilator responses to acetylcholine induced by IMQ microbiota were normalized after interleukin-17 neutralization. In conclusion, gut microbiota plays a role in the TLR7-driven increase in Th17 cell, endothelial dysfunction, vascular inflammation, and hypertension. The vascular changes induced by IMQ microbiota were initiated by Th17 infiltrating the vasculature.
SN 2076-3921
YR 2021
FD 2021-09-07
LK https://hdl.handle.net/10668/28079
UL https://hdl.handle.net/10668/28079
LA en
DS RISalud
RD Apr 5, 2025