RT Journal Article
T1 Isolation and characterization of myogenic precursor cells from human cremaster muscle.
A1 Naldaiz-Gastesi, Neia
A1 Goicoechea, Maria
A1 Aragon, Isabel M-ª
A1 Perez-Lopez, Virginia
A1 Fuertes-Alvarez, Sandra
A1 Herrera-Imbroda, Bernardo
A1 Lopez-de-Munain, Adolfo
A1 de-Luna-Diaz, Resi
A1 Baptista, Pedro M
A1 Fernandez, M Alejandro
A1 Lara, Maria Fernanda
A1 Izeta, Ander
K1 Skeletal muscle
K1 Cremaster muscle
K1 Mesenchymal stem cells
K1 Myogenic precursor cells
K1 Satellite cells
K1 Muscle regeneration
K1 Cell culture
K1 Cell therapy
K1 Stem cell transplantation
K1 Urinary incontinence
AB Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy.
PB Nature Publishing Group
YR 2019
FD 2019-03-05
LK http://hdl.handle.net/10668/13662
UL http://hdl.handle.net/10668/13662
LA en
NO Naldaiz-Gastesi N, Goicoechea M, Aragón IM, Pérez-López V, Fuertes-Alvarez S, et al. Isolation and characterization of myogenic precursor cells from human cremaster muscle. Sci Rep. 2019 Mar 5;9(1):3454.
DS RISalud
RD Oct 6, 2025