RT Journal Article
T1 A Dereplication and Bioguided Discovery Approach to Reveal New Compounds from a Marine-Derived Fungus Stilbella fimetaria
A1 Kildgaard, Sara
A1 Subko, Karolina
A1 Phillips, Emma
A1 Goidts, Violaine
A1 de la Cruz, Mercedes
A1 Diaz, Caridad
A1 Gotfredsen, Charlotte H.
A1 Andersen, Birgitte
A1 Frisvad, Jens C.
A1 Nielsen, Kristian F.
A1 Larsen, Thomas O.
K1 bioguided-discovery
K1 dereplication
K1 cytotoxicity
K1 antifungal
K1 MS/HRMS
K1 marine-derived
K1 pimarane-type diterpenoids
K1 ilicicolin H
K1 Antifungal ilicicolin h
K1 Secondary metabolites
K1 Molecular networking
K1 Natural-products
K1 Myrothecium-verrucaria
K1 Bioactive compounds
K1 Myrocin-b
K1 Antibiotics
K1 Antiamebins
K1 Diversity
AB A marine-derived Stilbella fimetaria fungal strain was screened for new bioactive compounds based on two different approaches: (i) bio-guided approach using cytotoxicity and antimicrobial bioassays; and (ii) dereplication based approach using liquid chromatography with both diode array detection and high resolution mass spectrometry. This led to the discovery of several bioactive compound families with different biosynthetic origins, including pimarane-type diterpenoids and hybrid polyketide-non ribosomal peptide derived compounds. Prefractionation before bioassay screening proved to be a great aid in the dereplication process, since separate fractions displaying different bioactivities allowed a quick tentative identification of known antimicrobial compounds and of potential new analogues. A new pimarane-type diterpene, myrocin F, was discovered in trace amounts and displayed cytotoxicity towards various cancer cell lines. Further media optimization led to increased production followed by the purification and bioactivity screening of several new and known pimarane-type diterpenoids. A known broad-spectrum antifungal compound, ilicicolin H, was purified along with two new analogues, hydroxyl-ilicicolin H and ilicicolin I, and their antifungal activity was evaluated.
PB Mdpi ag
SN 1660-3397
YR 2017
FD 2017-08-01
LK https://hdl.handle.net/10668/24599
UL https://hdl.handle.net/10668/24599
LA en
DS RISalud
RD Apr 7, 2025