RT Journal Article
T1 One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from Streptomyces cacaoi CA-170360.
A1 Román-Hurtado, Fernando
A1 Sánchez-Hidalgo, Marina
A1 Martín, Jesús
A1 Ortiz-López, Francisco Javier
A1 Carretero-Molina, Daniel
A1 Reyes, Fernando
A1 Genilloud, Olga
K1 BE-18257
K1 Cas9-assisted targeting of chromosome segments (CATCH) cloning
K1 Streptomyces
K1 heterologous expression
K1 non-ribosomal peptide synthetases (NRPS)
K1 pentaminomycin
K1 secondary metabolites
AB The strain Streptomyces cacaoi CA-170360 produces the cyclic pentapeptides pentaminomycins A-H and BE-18257 A-C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the non-ribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.
SN 2076-2607
YR 2021
FD 2021-01-08
LK http://hdl.handle.net/10668/16955
UL http://hdl.handle.net/10668/16955
LA en
DS RISalud
RD Apr 18, 2025