RT Journal Article
T1 Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser
A1 Illescas-Montes, Rebeca
A1 Melguizo-Rodriguez, Lucia
A1 Javier Manzano-Moreno, Francisco
A1 Garcia-Martinez, Olga
A1 Ruiz, Concepcion
A1 Ramos-Torrecillas, Javier
K1 wound healing
K1 fibroblasts
K1 diode laser
K1 biostimulation
K1 cell culture
K1 Platelet-rich plasma
K1 Clinical-implications
K1 Cell-proliferation
K1 Pressure ulcers
K1 Skin wounds
K1 Therapy
K1 Osteoblast
K1 Differentiation
K1 Tissue
K1 Photobiomodulation
AB Background: Fibroblasts are the main cells involved in regeneration during wound healing. The objective was to determine the effect of 940 nm diode laser on cultured human fibroblasts using different irradiation regimens. Methods: The CCD-1064Sk human epithelial fibroblast cell line was treated with a 940 nm diode laser at different energy doses (power: 0.2-1 W and energy density: 1-7 J/cm(2)) using different transmission modes (continuous or pulsed). The effect on cell growth at 24 and 72 h post-treatment was examined by measuring the proliferative capacity, the impact on the cell cycle, and the effect on cell differentiation. Results: fibroblast proliferative capacity was increased at 24 and 72 h post-treatment as a function of the energy dose. The greatest increase was observed with a power of 0.2 or 0.5 W and energy density between 1 and 4 J/cm(2); no difference was observed between continuous and pulsed modes. There were no significant differences in cell cycle between treated groups and controls. alpha-actin expression was increased by treatment, indicating enhanced cell differentiation. Conclusion: The 940 nm diode laser has biostimulating effects on fibroblasts, stimulating proliferative capacity and cell differentiation without altering the cell cycle. Further researches are necessary to explore its potential clinical usefulness in wound healing.
PB MDPI AG
SN 1996-1944
YR 2017
FD 2017-07-07
LK http://hdl.handle.net/10668/19290
UL http://hdl.handle.net/10668/19290
LA en
NO Illescas-Montes R, Melguizo-Rodríguez L, Manzano-Moreno FJ, García-Martínez O, Ruiz C, Ramos-Torrecillas J. Cultured Human Fibroblast Biostimulation Using a 940 nm Diode Laser. Materials (Basel). 2017 Jul 13;10(7):793
DS RISalud
RD Apr 17, 2025