RT Journal Article
T1 Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples
A1 Soriano-Lerma, Ana
A1 Pérez-Carrasco, Virginia
A1 Sánchez-Marañón, Manuel
A1 Ortiz-González, Matilde
A1 Sánchez-Martín, Victoria
A1 Gijón, Juan
A1 Navarro-Mari, José María
A1 García-Salcedo, José Antonio
A1 Soriano, Miguel
K1 RNA, ribosomal, 16S
K1 Saliva
K1 Microbiota
K1 Soil
K1 Bacteria
K1 Biomarkers
K1 ARN ribosómico 16S
K1 Suelo
K1 Bacterias
K1 Biomarcadores
AB Next generation sequencing methods are widely used in evaluating the structure and functioning of microbial communities, especially those centered on 16S rRNA subunit. Since Illumina Miseq, the most used sequencing platform, does not allow the full sequencing of 16S rRNA gene, this study aims to evaluate whether the choice of different target regions might affect the outcome of microbiome studies regarding soil and saliva samples. V1V3, V3V4, V4V5 and V6V8 domains were studied, finding that while some regions showed differences in the detection of certain bacterial taxa and in the calculation of alpha diversity, especially in soil samples, the overall effect did not compromise the differentiation of any sample type in terms of taxonomic analysis at the genus level. 16S rRNA target regions did affect the detection of specific bacteria related to soil quality and development, and microbial genera used as health biomarkers in saliva. V1V3 region showed the closest similarity to internal sequencing control mock community B, suggesting it might be the most preferable choice regarding data reliability.
PB Springer Nature
YR 2020
FD 2020-08-12
LK http://hdl.handle.net/10668/4402
UL http://hdl.handle.net/10668/4402
LA en
NO Soriano-Lerma A, Pérez-Carrasco V, Sánchez-Marañón M, Ortiz-González M, Sánchez-Martín V, Gijón J, et al. Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples. Sci Rep. 2020 Aug 12;10(1):13637
DS RISalud
RD Apr 8, 2025