RT Journal Article
T1 The Impact of Melatonin and NLRP3 Inflammasome on the Expression of microRNAs in Aged Muscle
A1 Sayed, Ramy KA
A1 Fernández-Ortiz, Marisol
A1 Fernández-Martínez, José
A1 Aranda Martínez, Paula
A1 Guerra-Librero, Ana
A1 Rodríguez-Santana, César
A1 de Haro, Tomás
A1 Escames, Germaine
A1 Acuña-Castroviejo, Darío
A1 Rusanova, Iryna
K1 microRNAs
K1 Melatonin
K1 NLRP3 inflammasome
K1 NF-kB
K1 Aging
K1 Pro-caspase-1
K1 Caspase-3
K1 bcl-2
K1 Muscle fibers
K1 Collagen
K1 MicroARNs
K1 Melatonina
K1 Proteína con dominio pirina 3 de la familia NLR
K1 FN-kappa B
K1 Envejecimiento
K1 Caspasa 1
K1 Caspasa 3
K1 Fibras Musculares Esqueléticas
AB Muscular aging is a complex process and underlying physiological mechanisms are not fully clear. In recent years, the participation of the NF-kB pathway and the NLRP3 inflammasome in the chronic inflammation process that accompanies the skeletal muscle's aging has been confirmed. microRNAs (miRs) form part of a gene regulatory machinery, and they control numerous biological processes including inflammatory pathways. In this work, we studied the expression of four miRs; three of them are considered as inflammatory-related miRs (miR-21, miR-146a, and miR-223), and miR-483, which is related to the regulation of melatonin synthesis, among other targets. To investigate the changes of miRs expression in muscle along aging, the impact of inflammation, and the role of melatonin in aged skeletal muscle, we used the gastrocnemius muscle of wild type (WT) and NLRP3-knockout (NLRP3-) mice of 3, 12, and 24 months-old, with and without melatonin supplementation. The expression of miRs and pro-caspase-1, caspase-3, pro-IL-1β, bax, bcl-2, and p53, was investigated by qRT-PCR analysis. Histological examination of the gastrocnemius muscle was also done. The results showed that age increased the expression of miR-21 (p < 0.01), miR-146a, and miR-223 (p < 0.05, for both miRs) in WT mice, whereas the 24-months-old mutant mice revealed decline of miR-21 and miR-223 (p < 0.05), compared to WT age. The lack of NLRP3 inflammasome also improved the skeletal muscle fibers arrangement and reduced the collagen deposits compared with WT muscle during aging. For the first time, we showed that melatonin significantly reduced the expression of miR-21, miR-146a, and miR-223 (p < 0.05 for all ones, and p < 0.01 for miR-21 at 24 months old) in aged WT mice, increased miR-223 in NLRP3- mice (p < 0.05), and induced miR-483 expression in both mice strains, this increase being significant at 24 months of age.
PB MDPI
YR 2021
FD 2021-03-27
LK http://hdl.handle.net/10668/4130
UL http://hdl.handle.net/10668/4130
LA en
NO Sayed RK, Fernández-Ortiz M, Fernández-Martínez J, Aranda Martínez P, Guerra-Librero A, Rodríguez-Santana C, et al. The Impact of Melatonin and NLRP3 Inflammasome on the Expression of microRNAs in Aged Muscle. Antioxidants (Basel). 2021 Mar 27;10(4):524
DS RISalud
RD Apr 7, 2025