RT Journal Article
T1 Metallo-Liposomes Derived from the [Ru(bpy)(3)](2+) Complex as Nanocarriers of Therapeutic Agents
A1 Moya, Maria Luisa
A1 Ostos, Francisco Jose
A1 Moreno, Izamar
A1 Garcia, Diandra
A1 Moreno-Gordillo, Paula
A1 V. Rosado, Ivan
A1 Lopez-Cornejo, Pilar
A1 Lebron, Jose Antonio
A1 Lopez-Lopez, Manuel
K1 metallo-liposome
K1 nanocarrier
K1 gene therapy
K1 DNA
K1 doxorubicin
K1 TEM
K1 sensor
K1 Drug-delivery
K1 Doxorubicin
K1 Encapsulation
K1 Metallosurfactants
K1 Vesicles
AB The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)(3)](2+)) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy -TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established.
PB Mdpi
YR 2021
FD 2021-05-01
LK http://hdl.handle.net/10668/19254
UL http://hdl.handle.net/10668/19254
LA en
DS RISalud
RD Apr 11, 2025