RT Journal Article
T1 Deep Phenotypic Characterisation of CTCs by Combination of Microfluidic Isolation (IsoFlux) and Imaging Flow Cytometry (ImageStream).
A1 Ruiz-Rodríguez, Antonio J
A1 Molina-Vallejo, Maria P
A1 Aznar-Peralta, Inés
A1 González Puga, Cristina
A1 Cañas García, Inés
A1 González, Encarna
A1 Lorente, Jose A
A1 Serrano, M Jose
A1 Garrido-Navas, M Carmen
K1 CRC
K1 CTC heterogeneity
K1 ImageStream
K1 IsoFlux
K1 circulating tumour cells
AB The isolation of circulating tumour cells (CTCs) in colorectal cancer (CRC) mostly relies on the expression of epithelial markers such as EpCAM, and phenotypic characterisation is usually performed under fluorescence microscopy with only one or two additional markers. This limits the ability to detect different CTC subpopulations based on multiple markers. The aim of this work was to develop a novel protocol combining two platforms (IsoFluxTM and ImageStream®X) to improve CTC evaluation. Cancer cell lines and peripheral blood from healthy donors were used to evaluate the efficiency of each platform independently and in combination. Peripheral blood was extracted from 16 early CRC patients (before loco-regional surgery) to demonstrate the suitability of the protocol for CTC assessment. Additionally, peripheral blood was extracted from nine patients one month after surgery to validate the utility of our protocol for identifying CTC subpopulation changes over time. Results: Our protocol had a mean recovery efficiency of 69.5% and a limit of detection of at least four cells per millilitre. We developed an analysis method to reduce noise from magnetic beads used for CTC isolation. CTCs were isolated from CRC patients with a median of 37 CTCs (IQ 13.0-85.5) at baseline. CTCs from CRC patients were significantly (p
SN 2072-6694
YR 2021
FD 2021-12-20
LK https://hdl.handle.net/10668/26133
UL https://hdl.handle.net/10668/26133
LA en
DS RISalud
RD Apr 8, 2025