RT Journal Article
T1 Expression of the transcription factor NFAT2 in human neutrophils: IgE-dependent, Ca2+- and calcineurin-mediated NFAT2 activation.
A1 Vega, Antonio
A1 Chacón, Pedro
A1 Monteseirín, Javier
A1 El Bekay, Rajaa
A1 Alba, Gonzalo
A1 Martín-Nieto, José
A1 Sobrino, Francisco
K1 Neutrophils
K1 NFAT
K1 Calcineurin
K1 Allergy
K1 Ciclooxigenasa 2
K1 Activación Neutrófila
K1 Transcripción Genética
K1 Inmunoglobulina E
K1 Núcleo celular
K1 Regulación de la expresión génica
K1 Calcio
K1 Factores de transcripción NFATC
AB NFAT (nuclear factors of activated T cells) proteins constitute a family of transcription factors involved in mediating signal transduction. The presence of NFAT isoforms has been described in all cell types of the immune system, with the exception of neutrophils. In the present work we report for the first time the expression in human neutrophils of NFAT2 mRNA and protein. We also report that specific antigens were able to promote NFAT2 protein translocation to the nucleus, an effect that was mimicked by the treatment of neutrophils with anti-immunoglobulin E (anti-IgE) or anti-Fcepsilon-receptor antibodies. Antigens, anti-IgE and anti-FcepsilonRs also increased Ca2+ release and the intracellular activity of calcineurin, which was able to interact physically with NFAT2, in parallel to eliciting an enhanced NFAT2 DNA-binding activity. In addition, specific chemical inhibitors of the NFAT pathway, such as cyclosporin A and VIVIT peptide, abolished antigen and anti-IgE-induced cyclooxygenase-2 (COX2) gene upregulation and prostaglandin (PGE(2)) release, suggesting that this process is through NFAT. Our results provide evidence that NFAT2 is constitutively expressed in human neutrophils, and after IgE-dependent activation operates as a transcription factor in the modulation of genes, such as COX2, during allergic inflammation.
PB Company of Biologists
SN 0021-9533
YR 2007
FD 2007-07-15
LK http://hdl.handle.net/10668/1343
UL http://hdl.handle.net/10668/1343
LA en
NO Vega A, Chacón P, Monteseirín J, El Bekay R, Alba G, Martín-Nieto J, et al. Expression of the transcription factor NFAT2 in human neutrophils: IgE-dependent, Ca2+- and calcineurin-mediated NFAT2 activation. J. Cell. Sci.. 2007; 120(Pt 14):2328-37
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 17, 2025