RT Journal Article
T1 Exchange of cellular components between platelets and tumor cells: impact on tumor cells behavior.
A1 Rodriguez-Martinez, Alba
A1 Simon-Saez, Iris
A1 Perales, Sonia
A1 Garrido-Navas, Carmen
A1 Russo, Alessandro
A1 de Miguel-Perez, Diego
A1 Puche-Sanz, Ignacio
A1 Alaminos, Clara
A1 Ceron, Jorge
A1 Lorente, Jose A
A1 Molina, Maria Pilar
A1 Gonzalez, Coral
A1 Cristofanilli, Massimo
A1 Ortigosa-Palomo, Alba
A1 Real, Pedro J
A1 Rolfo, Christian
A1 Serrano, María J
K1 CD61
K1 Platelets
K1 circulating tumor cells (CTCs)
K1 epithelial-to-mesenchymal transition (EMT)
K1 platelet-educated tumors (PETs)
AB Background: Platelets are active players in tumorigenesis, although the exact interactive mechanisms and their direct impact on tumor cells remain largely unknown. Methods: Bidirectional transference of lipids, proteins and RNA between platelets and tumor cells and its impact on tumor cell behavior and tumor process are analyzed in this work. Phenotypic, genetic and functional modifications induced by platelets were analyzed both in tumor cell lines and in circulating tumor cells (CTCs). Results: Data from these assays showed that platelets transferred structural components to tumor cells with higher efficiency than tumor cells to platelets (p = 0.001). This biological interplay occurred by direct contact, internalization or via extracellular vesicles. As a result, tumor cells acquired platelet markers (CD61 and CD42), showed decreased EpCAM, expressed epithelial-to-mesenchymal transition markers, and increased proliferation rates. Moreover, we were able to detect CD61 in CTCs from early and advanced prostate cancer. Conclusions: Our results demonstrated, for the first time, that platelets educate tumor cells by highly efficient transference of lipids, proteins and RNA through different mechanisms. These results suggest that tumor cells and CTCs might acquire highly dynamic and aggressive phenotypes due to platelets interaction including EMT, stem-like phenotype and high proliferative rates.
PB Ivyspring International Publisher
YR 2022
FD 2022-12-21
LK http://hdl.handle.net/10668/21671
UL http://hdl.handle.net/10668/21671
LA en
NO Rodriguez-Martinez A, Simon-Saez I, Perales S, Garrido-Navas C, Russo A, et al. Exchange of cellular components between platelets and tumor cells: impact on tumor cells behavior. Theranostics. 2022 Feb 7;12(5):2150-2161.
NO The authors thank all patients and donors who contributed to the study. This work was supported by ISCIII Health Research Institute (P17/00989), 'La Caixa' Foundation, AR-M was supported by PhD program from Ministry of Education Spanish Government (FPU14/05461), IS-S was supported by European Youth Guarantee.
DS RISalud
RD Apr 10, 2025