RT Journal Article
T1 Novel and Conventional Isolation Techniques to Obtain Planctomycetes from Marine Environments.
A1 Vitorino, Inês
A1 Santos, José Diogo Neves
A1 Godinho, Ofélia
A1 Vicente, Francisca
A1 Vasconcelos, Vítor
A1 Lage, Olga Maria
K1 Planctomycetia
K1 ecology
K1 iChip
K1 invertebrates
K1 macroalgae
K1 novel taxa
K1 sediments
AB Bacteria from the distinctive Planctomycetes phylum are well spread around the globe; they are capable of colonizing many habitats, including marine, freshwater, terrestrial, and even extreme habitats such as hydrothermal vents and hot springs. They can also be found living in association with other organisms, such as macroalgae, plants, and invertebrates. While ubiquitous, only a small fraction of the known diversity includes axenic cultures. In this study, we aimed to apply conventional techniques to isolate, in diverse culture media, planctomycetes from two beaches of the Portuguese north-coast by using sediments, red, green, and brown macroalgae, the shell of the mussel Mytilus edulis, an anemone belonging to the species Actinia equina, and seawater as sources. With this approach, thirty-seven isolates closely related to seven species from the families Planctomycetaceae and Pirellulaceae (class Planctomycetia) were brought into pure culture. Moreover, we applied an iChip inspired in-situ culturing technique to successfully retrieve planctomycetes from marine sediments, which resulted in the isolation of three additional strains, two affiliated to the species Novipirellula caenicola and one to a putative novel Rubinisphaera. This work enlarges the number of isolated planctomycetal strains and shows the adequacy of a novel methodology for planctomycetes isolation.
SN 2076-2607
YR 2021
FD 2021-10-01
LK https://hdl.handle.net/10668/24601
UL https://hdl.handle.net/10668/24601
LA en
DS RISalud
RD Apr 11, 2025