RT Journal Article
T1 Evolution of Energy Related Metabolites in Plasma from Newborns with Hypoxic-Ischemic Encephalopathy during Hypothermia Treatment.
A1 Sanchez-Illana, Angel
A1 Nuñez-Ramiro, Antonio
A1 Cernada, María
A1 Parra-Llorca, Anna
A1 Valverde, Eva
A1 Blanco, Dorotea
A1 Moral-Pumarega, Maria Teresa
A1 Cabañas, Fernando
A1 Boix, Hector
A1 Pavon, Antonio
A1 Chaffanel, Mercedes
A1 Benavente-Fernandez, Isabel
A1 Tofe, Ines
A1 Loureiro, Begoña
A1 Fernandez-Lorenzo, José R
A1 Fernandez-Colomer, Belen
A1 Garcia-Robles, Ana
A1 Kuligowski, Julia
A1 Vento, Maximo
A1 HYPOTOP Study Group, 
K1 Pyruvic Acid
K1 Lactic Acid
K1 Metabolism
K1 Mass Spectrometry
K1 Chromatography, Gas
AB Therapeutic hypothermia (TH) initiated within 6 h from birth is the most effective therapeutic approach for moderate to severe hypoxic-ischemic encephalopathy (HIE). However, underlying mechanisms and effects on the human metabolism are not yet fully understood. This work aims at studying the evolution of several energy related key metabolites in newborns with HIE undergoing TH employing gas chromatography - mass spectrometry. The method was validated following stringent FDA requirements and applied to 194 samples from a subgroup of newborns with HIE (N = 61) enrolled in a multicenter clinical trial (HYPOTOP) for the determination of lactate, pyruvate, ketone bodies and several Krebs cycle metabolites at different sampling time points. The analysis of plasma samples from newborns with HIE revealed a decrease of lactate, pyruvate and β-hydroxybutyrate concentrations, whereas rising malate concentrations were observed. In healthy control newborns (N = 19) significantly lower levels of pyruvate and lactate were found in comparison to age-matched newborns with HIE undergoing TH, whereas acetoacetate and β-hydroxybutyrate levels were clearly increased. Access to a validated analytical method and a controlled cohort of newborns with HIE undergoing hypothermia treatment for the first time allowed the in-depth study of the evolution of key metabolites of metabolic junctions in this special population.
PB Nature Publishing Group
YR 2017
FD 2017-12-06
LK http://hdl.handle.net/10668/11885
UL http://hdl.handle.net/10668/11885
LA en
NO Sánchez-Illana Á, Núñez-Ramiro A, Cernada M, Parra-Llorca A, Valverde E, Blanco D, et al.  Evolution of Energy Related Metabolites in Plasma from Newborns with Hypoxic-Ischemic Encephalopathy during Hypothermia Treatment. Sci Rep. 2017 Dec 6;7(1):17039
NO ANR acknowledges a research contract by the Spanish Maternal, Neonatal and Developmental Network SAMID RETICS funded by the PN 2018–2011 (Spain), ISCIII-Sub-Directorate General for Research Assessment and Promotion and the European Regional Development Fund (FEDER), reference RD12/0026. JK, MCe and ASI are grateful for their personal grants from the Instituto Carlos III (grant numbers CP16/00034, JR16/00038 and FI16/00380, respectively). APL acknowledges her personal grant from the Health Research Institute La Fe (CPR2015/0371).
DS RISalud
RD Apr 17, 2025