RT Journal Article
T1 A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner
A1 Ramírez-López, María Teresa
A1 Vázquez, Mariam
A1 Lomazzo, Ermelinda
A1 Hofmann, Clementine
A1 Blanco, Rosario Noemi
A1 Alén, Francisco
A1 Antón, María
A1 Decara, Juan
A1 Arco, Rocío
A1 Orio, Laura
A1 Suárez, Juan
A1 Lutz, Beat
A1 Gómez de Heras, Raquel
A1 Bindila, Laura
A1 Rodríguez de Fonseca, Fernando
K1 Desnutrición
K1 Embarazo
K1 Endocannabinoides
K1 Ácido Araquidónico
K1 Encéfalo
K1 Animales recién nacidos
K1 Ratas wistar
K1 Femenino
K1 Masculino
K1 Emociones
K1 Cognición
K1 Restricción calórica
K1 Hipotálamo
K1 Hipocampo
K1 Bulbo olfatorio
K1 Peso al nacer
AB Undernutrition during pregnancy has been associated to increased vulnerability to develop metabolic and behavior alterations later in life. The endocannabinoid system might play an important role in these processes. Therefore, we investigated the effects of a moderate maternal calorie-restricted diet on the levels of the endocannabinoid 2-arachidonoyl glycerol (2-AG), arachidonic acid (AA) and the N-acylethanolamines (NAEs) anandamide (AEA), oleoylethanolamide (OEA) and palmitoylethanolamide (PEA) in the brain of newborn rat offspring. We focused on brain structures involved in metabolism, feeding behavior, as well as emotional and cognitive responses. Female Wistar rats were assigned during the entire pregnancy to either control diet (C) or restriction diet (R), consisting of a 20% calorie-restricted diet. Weight gain and caloric intake of rat dams were monitored and birth outcomes were assessed. 2-AG, AA and NAE levels were measured in hypothalamus, hippocampus and olfactory bulb of the offspring. R dams displayed lower gain weight from the middle pregnancy and consumed less calories during the entire pregnancy. Offspring from R dams were underweight at birth, but litter size was unaffected. In hypothalamus, R male offspring displayed decreased levels of AA and OEA, with no change in the levels of the endocannabinoids 2-AG and AEA. R female exhibited decreased 2-AG and PEA levels. The opposite was found in the hippocampus, where R male displayed increased 2-AG and AA levels, and R female exhibited elevated levels of AEA, AA and PEA. In the olfactory bulb, only R female presented decreased levels of AEA, AA and PEA. Therefore, a moderate diet restriction during the entire pregnancy alters differentially the endocannabinoids and/or endocannabinoid-related lipids in hypothalamus and hippocampus of the underweight offspring, similarly in both sexes, whereas sex-specific alterations occur in the olfactory bulb. Consequently, endocannabinoid and endocannabinoid-related lipid signaling alterations might be involved in the long-term and sexual dimorphism effects commonly observed after undernutrition and low birth weight.
PB Public Libray of Science
YR 2017
FD 2017-03-27
LK http://hdl.handle.net/10668/2687
UL http://hdl.handle.net/10668/2687
LA en
NO Ramírez-López MT,Vázquez M, Lomazzo E, Hofmann C, Blanco RN, Alén F, et al. A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. Plos One. 2017;12(3):e0174307.
NO Existe para su consulta material suplementario aportado por el editor.
DS RISalud
RD Apr 7, 2025