RT Journal Article
T1 Identification of the cellular components involved in de novo immune hepatitis: a quantitative immunohistochemical analysis.
A1 Aguado-Domínguez, Elena
A1 Gómez, Lourdes
A1 Sousa, José Manuel
A1 Gómez-Bravo, Miguel Ángel
A1 Núñez-Roldán, Antonio
A1 Aguilera, Isabel
K1 Chronic rejection
K1 De novo immune hepatitis
K1 Donor/recipient mismatch
K1 GSTT1
K1 Inflammatory infiltrates
K1 Liver biopsy
K1 newCAST
AB Diagnosis of de novo immune hepatitis (dnIH) after liver transplantation relies on biopsy findings, with an abundance of plasma cells (PCs) in the inflammatory infiltrates a hallmark of the disease. Very little is known about what other types of immune cells exist in the infiltrates mainly located in the portal areas of the liver tissue. We analyzed the composition of T cells, B cells, PCs, and macrophages in the liver biopsies of 12 patients with dnIH, 9 of them obtained at the time of diagnosis. For comparison, biopsies from 9 patients with chronic rejection (CR) were included in the study. The results were analyzed by a computer-assisted stereology quantification method. The major components of the infiltrates in the portal areas were CD3+ T lymphocytes in both groups, with 36.6% in the dnIH group versus 49.4% in the CR group. CD20+ B lymphocytes represented 14.9% in the dnIH group and 29.1% in the CR group. Macrophage levels were very similar in the dnIH and CR group (19.7% versus 16.8%, respectively). PCs were much less represented in CR biopsies than those from the dnIH group (mean value of 4.7% versus 28.8%). In conclusion, the determination of a characteristic cellular profile could be an important tool for a more reliable diagnosis of dnIH, in support of the histological evaluation made by the pathologist, which in most cases is challenging. Recognition of this condition is crucial because it leads to graft failure if left untreated.
YR 2018
FD 2018-03-13
LK http://hdl.handle.net/10668/12237
UL http://hdl.handle.net/10668/12237
LA en
DS RISalud
RD Apr 20, 2025