RT Journal Article
T1 Rationale for the Use of Radiation-Activated Mesenchymal Stromal/Stem Cells in Acute Respiratory Distress Syndrome
A1 Tovar, Isabel
A1 Guerrero, Rosa
A1 López-Peñalver, Jesús J.
A1 Expósito, José
A1 Ruiz de Almodóvar, José Mariano
K1 Experimental radiotherapy
K1 Radiobiology
K1 Mesenchymal stem cells
K1 Cell therapy
K1 Exosome
K1 Annexin A1
K1 Acute respiratory-distress syndrome
K1 COVID-19
K1 Radiobiología
K1 Células madre mesenquimatosas
K1 Tratamiento basado en trasplante de células y tejidos
K1 Exosomas
K1 Anexina A1
K1 Síndrome de dificultad respiratoria del adulto
AB We have previously shown that the combination of radiotherapy with human umbilical-cord-derived mesenchymal stromal/stem cells (MSCs) cell therapy significantly reduces the size of the xenotumors in mice, both in the directly irradiated tumor and in the distant nonirradiated tumor or its metastasis. We have also shown that exosomes secreted from MSCs preirradiated with 2 Gy are quantitatively, functionally and qualitatively different from the exosomes secreted from nonirradiated mesenchymal cells, and also that proteins, exosomes and microvesicles secreted by MSCs suffer a significant change when the cells are activated or nonactivated, with the amount of protein present in the exosomes of the preirradiated cells being 1.5 times greater compared to those from nonirradiated cells. This finding correlates with a dramatic increase in the antitumor activity of the radiotherapy when is combined with MSCs or with preirradiated mesenchymal stromal/stem cells (MSCs*). After the proteomic analysis of the load of the exosomes released from both irradiated and nonirradiated cells, we conclude that annexin A1 is the most important and significant difference between the exosomes released by the cells in either status. Knowing the role of annexin A1 in the control of hypoxia and inflammation that is characteristic of acute respiratory-distress syndrome (ARDS), we designed a hypothetical therapeutic strategy, based on the transplantation of mesenchymal stromal/stem cells stimulated with radiation, to alleviate the symptoms of patients who, due to pneumonia caused by SARS-CoV-2, require to be admitted to an intensive care unit for patients with life-threatening conditions. With this hypothesis, we seek to improve the patients' respiratory capacity and increase the expectations of their cure.
PB MDPI
YR 2020
FD 2020-09-02
LK http://hdl.handle.net/10668/3391
UL http://hdl.handle.net/10668/3391
LA en
NO Tovar I, Guerrero R, López-Peñalver JJ, Expósito J, Ruiz de Almodóvar JM. Rationale for the Use of Radiation-Activated Mesenchymal Stromal/Stem Cells in Acute Respiratory Distress Syndrome. Cells. 2020 Sep 2;9(9):2015.
DS RISalud
RD Apr 6, 2025