RT Journal Article
T1 Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges.
A1 López-Longarela, Barbara
A1 Morrison, Emma E
A1 Tranter, John D
A1 Chahman-Vos, Lianne
A1 Léonard, Jean-François
A1 Gautier, Jean-Charles
A1 Laurent, Sébastien
A1 Lartigau, Aude
A1 Boitier, Eric
A1 Sautier, Lucile
A1 Carmona-Saez, Pedro
A1 Martorell-Marugan, Jordi
A1 Mellanby, Richard J
A1 Pernagallo, Salvatore
A1 Ilyine, Hugh
A1 Rissin, David M
A1 Duffy, David C
A1 Dear, James W
A1 Díaz-Mochón, Juan J
AB Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10 μL of serum or plasma without any extraction steps, with a limit of detection of 1.32 pM that enabled the identification of DILI. Testing of 192 human serum samples showed that DCL accurately identified patients at risk of DILI after acetaminophen overdose (area under ROC curve 0.98 (95% CI; 0.96-1), P
YR 2020
FD 2020-01-27
LK https://hdl.handle.net/10668/28411
UL https://hdl.handle.net/10668/28411
LA en
DS RISalud
RD Apr 5, 2025