RT Journal Article
T1 A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering.
A1 Garzón, Ingrid
A1 Carriel, Victor
A1 Marín-Fernández, Ana Belén
A1 Oliveira, Ana Celeste
A1 Garrido-Gómez, Juan
A1 Campos, Antonio
A1 Sánchez-Quevedo, María Del Carmen
A1 Alaminos, Miguel
K1 Disco de la Articulación Temporomandibular
K1 Condrocitos
K1 Trasplante Autólogo
K1 Células Cultivadas
K1 Ingeniería de Tejidos
K1 Expresión Génica
K1 Supervivencia Celular
K1 Proteínas Reguladoras de la Apoptosis
AB Temporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5-P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5-P6 for cell therapy protocols.
PB Public Library of Science
PB Nuno M. Neves, University of Minho, Portugal
YR 2012
FD 2012-12-18
LK http://hdl.handle.net/10668/780
UL http://hdl.handle.net/10668/780
LA en
NO Garzón I, Carriel V, Marín-Fernández AB, Oliveira AC, Garrido-Gómez J, Campos A, et al. A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering. PLoS ONE. 2012; 7(12):e51961
NO Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 19, 2025