RT Journal Article
T1 Polycomb regulation is coupled to cell cycle transition in pluripotent stem cells
A1 Asenjo, Helena G.
A1 Gallardo, Amador
A1 López-Onieva, Lourdes
A1 Tejada, Irene
A1 Martorell-Marugán, Jordi
A1 Carmona-Sáez, Pedro
A1 Landeira, David
K1 Polycomb Repressive Complex 2
K1 Mouse Embryonic Stem Cells
K1 Pluripotent Stem Cells
K1 Cell Cycle
K1 Chromatin
K1 Mice
K1 Genes
K1 Complejo Represivo Polycomb 2
K1 Células Madre Embrionarias de Ratones
K1 Células Madre Pluripotentes
K1 Ciclo Celular
K1 Cromatina
K1 Ratones
AB When self-renewing pluripotent cells receive a differentiation signal, ongoing cell duplication needs to be coordinated with entry into a differentiation program. Accordingly, transcriptional activation of lineage specifier genes and cell differentiation is confined to the G1 phase of the cell cycle by unknown mechanisms. We found that Polycomb repressive complex 2 (PRC2) subunits are differentially recruited to lineage specifier gene promoters across cell cycle in mouse embryonic stem cells (mESCs). Jarid2 and the catalytic subunit Ezh2 are markedly accumulated at target promoters during S and G2 phases, while the transcriptionally activating subunits EPOP and EloB are enriched during G1 phase. Fluctuations in the recruitment of PRC2 subunits promote changes in RNA synthesis and RNA polymerase II binding that are compromised in Jarid2 -/- mESCs. Overall, we show that differential recruitment of PRC2 subunits across cell cycle enables the establishment of a chromatin state that facilitates the induction of cell differentiation in G1 phase.
PB American Association for the Advancement of Science (AAAS)
YR 2020
FD 2020-03-04
LK http://hdl.handle.net/10668/3496
UL http://hdl.handle.net/10668/3496
LA en
NO Asenjo HG, Gallardo A, López-Onieva L, Tejada I, Martorell-Marugán J, Carmona-Sáez P, et al. Polycomb regulation is coupled to cell cycle transition in pluripotent stem cells. Sci Adv. 2020 Mar 4;6(10):eaay4768.
DS RISalud
RD Apr 7, 2025