RT Journal Article
T1 Glycosylated nanostructures in sublingual immunotherapy induce long-lasting tolerance in LTP allergy mouse model.
A1 Rodriguez, Maria J
A1 Ramos-Soriano, Javier
A1 Perkins, James R
A1 Mascaraque, Ainhoa
A1 Torres, Maria J
A1 Gomez, Francisca
A1 Diaz-Perales, Araceli
A1 Rojo, Javier
A1 Mayorga, Cristobalina
K1 Nanostructures
K1 Prunus persica
K1 Sublingual Immunotherapy
K1 T-Lymphocytes, Regulatory
AB An effective specific immunotherapy should contain elements to generate specific recognition (T-cell peptides) and to modulate the immunological response towards a Th1/Treg pattern by enhancing dendritic cells (DCs). We propose a novel sublingual immunotherapy for peach allergy, using systems, that combine Prup3-T-cell peptides with mannose dendrons (D1ManPrup3 and D4ManPrup3). Peach anaphylactic mice were treated 1, 2 and 5 nM concentrations. Tolerance was assessed one/five weeks after finishing treatment by determining in vivo/in vitro parameters after challenge with Prup3. Only mice receiving D1ManPrup3 at 2 nM were protected from anaphylaxis (no temperature changes, decrease in Prup3-sIgE and -sIgG1 antibody levels, and secreting cells) compared to PBS-treated mice. Moreover, an increase of Treg-cells and regulatory cytokines (IL-10+/IFN-γ+) in CD4+-T-cells and DCs were found. These changes were maintained at least five weeks after stopping treatment. D1ManPrup3 is an effective new approach of immunotherapy inducing protection from anaphylaxis which persists after finishing treatment.
PB Nature Publishing Group
YR 2019
FD 2019-03-11
LK http://hdl.handle.net/10668/13688
UL http://hdl.handle.net/10668/13688
LA en
NO Rodriguez MJ, Ramos-Soriano J, Perkins JR, Mascaraque A, Torres MJ, Gomez F, et al. Glycosylated nanostructures in sublingual immunotherapy induce long-lasting tolerance in LTP allergy mouse model. Sci Rep. 2019 Mar 11;9(1):4043. doi: 10.1038/s41598-019-40114-7. Erratum in: Sci Rep. 2020 Apr 17;10(1):6858
NO The study was funded by the Institute of Health “Carlos III” of the Ministry of Economy and Competitiveness: PI12/02481, PI15/00559 and PI18/00288, RETICS ARADyAL (RD16/0006/0001, /0003, /0011) and Sara Borrell Program (CD14/00242). Andalusian Regional Ministry of Economy and Knowledge: CTS-7433), Nicolas Monardes Program (C-00442012 SAS2013) and the Spanish Ministry of Economy and Competitiveness (CTQ2014-52328-P, CTQ201786265-P and AGL2014-52395-C2-2) and a FPI grant (JRS). Grants were cofunded by the European Regional Development Fund (ERDF)
DS RISalud
RD Apr 7, 2025