RT Journal Article
T1 Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway
A1 López-Gómez, Carlos
A1 Santiago-Fernández, Concepción
A1 García-Serrano, Sara
A1 García-Escobar, Eva
A1 Gutiérrez-Repiso, Carolina
A1 Rodríguez-Díaz, Cristina
A1 Ho-Plágaro, Ailec
A1 Martín-Reyes, Flores
A1 Garrido-Sánchez, Lourdes
A1 Valdés, Sergio
A1 Rodríguez-Cañete, Alberto
A1 Rodríguez-Pacheco, Francisca
A1 García-Fuentes, Eduardo
K1 Morbid obesity
K1 Insulin resistance
K1 IRS1
K1 p85α
K1 p110β
K1 Palmitic acid
K1 Oleic acid
K1 Docosahexaenoic acid
K1 Linoleic acid
K1 Visceral adipose tissue
K1 Obesidad mórbida
K1 Resistencia a la insulina
K1 Ácido palmítico
K1 Ácido oléico
K1 Ácidos docosahexaenoicos
K1 Ácido linoleico
K1 Grasa intraabdominal
K1 Andalucía
AB The effects of different types of fatty acids on the gene expression of key players in the IRS1/PI3K signaling pathway have been poorly studied. Material and Methods: We analyzed IRS1, p85α, and p110β mRNA expression and the fatty acid composition of phospholipids in visceral adipose tissue from patients with morbid obesity and from non-obese patients. Moreover, we analyzed the expression of those genes in visceral adipocytes incubated with oleic, linoleic, palmitic and dosahexaenoic acids. Results: We found a reduced IRS1 expression in patients with morbid obesity, independent of insulin resistance, and a reduced p110β expression in those with lower insulin resistance. A positive correlation was found between p85α and stearic acid, and between IRS1 and p110β with palmitic and dosahexaenoic acid. In contrast, a negative correlation was found between p85α and oleic acid, and between IRS1 and p110β with linoleic, arachidonic and adrenic acid. Incubation with palmitic acid decreased IRS1 expression. p85α was down-regulated after incubation with oleic and dosahexaenoic acid and up-regulated with palmitic acid. p110β expression was increased and decreased after incubation with oleic and palmitic acid, respectively. The ratio p85α/p110β was decreased by oleic and dosahexaenoic acid and increased by palmitic acid. Conclusions: Our in vitro results suggest a detrimental role of palmitic acid on the expression of gene related to insulin signaling pathway, with oleic acid being the one with the higher and more beneficial effects. DHA had a slight beneficial effect. Fatty acid-induced regulation of genes related to the IRS1/PI3K pathway may be a novel mechanism by which fatty acids regulate insulin sensitivity in visceral adipocytes.
PB MDPI
YR 2020
FD 2020-08-12
LK http://hdl.handle.net/10668/4103
UL http://hdl.handle.net/10668/4103
LA en
NO López-Gómez C, Santiago-Fernández C, García-Serrano S, García-Escobar E, Gutiérrez-Repiso C, Rodríguez-Díaz C, et al. Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway. J Clin Med. 2020 Aug 12;9(8):2615
DS RISalud
RD Apr 6, 2025