RT Generic
T1 Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene.
A1 Gil-Galvez, Alejandro
A1 Carbonell-Corvillo, Pilar
A1 Paradas, Carmen
A1 Miranda-Vizuete, Antonio
K1 Caenorhabditis elegans
K1 MYH7
K1 UNC-54
K1 mutation
K1 myosin
K1 overexpression
AB Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations into the unc-54 gene, the worm MYH7 ortholog. We report here that the C. elegans model is not appropriate for such studies if they involve expression of the UNC-54 protein (wild-type or fused to green fluorescent protein) above endogenous levels.
PB Future Science Ltd.
YR 2020
FD 2020-04-16
LK http://hdl.handle.net/10668/15384
UL http://hdl.handle.net/10668/15384
LA en
NO Gil-Gálvez A, Carbonell-Corvillo P, Paradas C, Miranda-Vizuete A. Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene. Biotechniques. 2020 Jun;68(6):296-299.
DS RISalud
RD Apr 4, 2025