RT Journal Article
T1 Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience.
A1 De Sousa, Paul A
A1 Steeg, Rachel
A1 Wachter, Elisabeth
A1 Bruce, Kevin
A1 King, Jason
A1 Hoeve, Marieke
A1 Khadun, Shalinee
A1 McConnachie, George
A1 Holder, Julie
A1 Kurtz, Andreas
A1 Seltmann, Stefanie
A1 Dewender, Johannes
A1 Reimann, Sascha
A1 Stacey, Glyn
A1 O'Shea, Orla
A1 Chapman, Charlotte
A1 Healy, Lyn
A1 Zimmermann, Heiko
A1 Bolton, Bryan
A1 Rawat, Trisha
A1 Atkin, Isobel
A1 Veiga, Anna
A1 Kuebler, Bernd
A1 Serano, Blanca Miranda
A1 Saric, Tomo
A1 Hescheler, Jürgen
A1 Brüstle, Oliver
A1 Peitz, Michael
A1 Thiele, Cornelia
A1 Geijsen, Niels
A1 Holst, Bjørn
A1 Clausen, Christian
A1 Lako, Majlinda
A1 Armstrong, Lyle
A1 Gupta, Shailesh K
A1 Kvist, Alexander J
A1 Hicks, Ryan
A1 Jonebring, Anna
A1 Brolén, Gabriella
A1 Ebneth, Andreas
A1 Cabrera-Socorro, Alfredo
A1 Foerch, Patrik
A1 Geraerts, Martine
A1 Stummann, Tina C
A1 Harmon, Shawn
A1 George, Carol
A1 Streeter, Ian
A1 Clarke, Laura
A1 Parkinson, Helen
A1 Harrison, Peter W
A1 Faulconbridge, Adam
A1 Cherubin, Luca
A1 Burdett, Tony
A1 Trigueros, Cesar
A1 Patel, Minal J
A1 Lucas, Christa
A1 Hardy, Barry
A1 Predan, Rok
A1 Dokler, Joh
A1 Brajnik, Maja
A1 Keminer, Oliver
A1 Pless, Ole
A1 Gribbon, Philip
A1 Claussen, Carsten
A1 Ringwald, Annette
A1 Kreisel, Beate
A1 Courtney, Aidan
A1 Allsopp, Timothy E
AB A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.
YR 2017
FD 2017-03-07
LK https://hdl.handle.net/10668/24717
UL https://hdl.handle.net/10668/24717
LA en
DS RISalud
RD Apr 5, 2025