RT Journal Article
T1 A High-Throughput Screening Platform of Microbial Natural Products for the Discovery of Molecules with Antibiofilm Properties against Salmonella.
A1 Paytubi, Sonia
A1 de La Cruz, Mercedes
A1 Tormo, Jose R
A1 Martín, Jesús
A1 González, Ignacio
A1 González-Menendez, Victor
A1 Genilloud, Olga
A1 Reyes, Fernando
A1 Vicente, Francisca
A1 Madrid, Cristina
A1 Balsalobre, Carlos
K1 HTS
K1 Salmonella
K1 antimicrobial
K1 biofilm
K1 natural products
K1 patulin
AB In this report, we describe a High-Throughput Screening (HTS) to identify compounds that inhibit biofilm formation or cause the disintegration of an already formed biofilm using the Salmonella Enteritidis 3934 strain. Initially, we developed a new methodology for growing Salmonella biofilms suitable for HTS platforms. The biomass associated with biofilm at the solid-liquid interface was quantified by staining both with resazurin and crystal violet, to detect living cells and total biofilm mass, respectively. For a pilot project, a subset of 1120 extracts from the Fundación MEDINA's collection was examined to identify molecules with antibiofilm activity. This is the first validated HTS assay of microbial natural product extracts which allows for the detection of four types of activities which are not mutually exclusive: inhibition of biofilm formation, detachment of the preformed biofilm and antimicrobial activity against planktonic cells or biofilm embedded cells. Currently, several extracts have been selected for further fractionation and purification of the active compounds. In one of the natural extracts patulin has been identified as a potent molecule with antimicrobial activity against both, planktonic cells and cells within the biofilm. These findings provide a proof of concept that the developed HTS can lead to the discovery of new natural compounds with antibiofilm activity against Salmonella and its possible use as an alternative to antimicrobial therapies and traditional disinfectants.
SN 1664-302X
YR 2017
FD 2017-03-02
LK http://hdl.handle.net/10668/10969
UL http://hdl.handle.net/10668/10969
LA en
DS RISalud
RD Apr 7, 2025