RT Journal Article
T1 Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections.
A1 Cano, Elena
A1 Carmona, Rita
A1 Ruiz-Villalba, Adrián
A1 Rojas, Anabel
A1 Chau, You-Ying
A1 Wagner, Kay D
A1 Wagner, Nicole
A1 Hastie, Nicholas D
A1 Muñoz-Chápuli, Ramón
A1 Pérez-Pomares, José M
K1 Gata4
K1 Wt1
K1 coronary endothelium
K1 proepicardium
K1 septum transversum
AB Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1(Cre)) and previously undescribed (G2-Gata4(Cre)) transgenic mouse models for the study of coronary vascular development, we show that extracardiac septum transversum/proepicardium (ST/PE)-derived endothelial cells are required for the formation of ventricular coronary arterio-venous vascular connections. Our results indicate that at least 20% of embryonic coronary arterial and capillary endothelial cells derive from the ST/PE compartment. Moreover, we show that conditional deletion of the ST/PE lineage-specific Wilms' tumor suppressor gene (Wt1) in the ST/PE of G2-Gata4(Cre) mice and in the endothelium of Tie2(Cre) mice disrupts embryonic coronary transmural patterning, leading to embryonic death. Taken together, our results demonstrate that ST/PE-derived endothelial cells contribute significantly to and are required for proper coronary vascular morphogenesis.
YR 2016
FD 2016-01-06
LK http://hdl.handle.net/10668/9709
UL http://hdl.handle.net/10668/9709
LA en
DS RISalud
RD Apr 17, 2025