RT Journal Article
T1 Personalized diagnostic approach and indirect quantification of extravasation in human anaphylaxis.
A1 Nuñez-Borque, Emilio
A1 Betancor, Diana
A1 Pastor-Vargas, Carlos
A1 Fernandez-Bravo, Sergio
A1 Martin-Blazquez, Ariadna
A1 Casado-Navarro, Natalia
A1 Lopez-Dominguez, David
A1 Gomez-Lopez, Alicia
A1 Rodriguez Del Rio, Pablo
A1 Tramon, Paloma
A1 Beitia, Juan Maria
A1 Moreno-Aguilar, Carmen
A1 Gonzalez-de-Olano, David
A1 Goikoetxea, María Jose
A1 Ibañez-Sandin, Maria Dolores
A1 Laguna, Jose Julio
A1 Cuesta-Herranz, Javier
A1 Esteban, Vanesa
K1 Anaphylaxis
K1 Haemoglobin
K1 Human serum albumin
K1 Protein concentration
K1 Tryptase
AB Anaphylaxis is the most acute and life-threatening manifestation of allergic disorders. Currently, there is a need to improve its medical management and increase the understanding of its molecular mechanisms. This study aimed to quantify the extravasation underlying human anaphylactic reactions and propose new theragnostic approaches. Molecular determinations were performed in paired serum samples obtained during the acute phase and at baseline from patients presenting with hypersensitivity reactions. These were classified according to their severity as Grades 1, 2 and 3, the two latter being considered anaphylaxis. Tryptase levels were measured by ImmunoCAP, and serum protein concentration was quantified by Bradford assay. Human serum albumin (HSA) and haemoglobin beta subunit (HBB) levels were determined by Western blot and polyacrylamide gel electrophoresis, respectively. A total of 150 patients were included in the study. Of them, 112 had experienced anaphylaxis (83 and 29 with Grade 2 and 3 reactions, respectively). Tryptase diagnostic efficiency substantially improved when considering patients' baseline values (33%-54%) instead of the acute value threshold (21%). Serum protein concentration and HSA significantly decreased in anaphylaxis (p  For the first time, the extravasation underlying human anaphylaxis has been evaluated based on the severity of the reaction using HSA and protein concentration measurements. Additionally, our findings propose new diagnostic and potential therapeutic approaches for this pathological event.
PB Wiley
YR 2022
FD 2022-06-07
LK http://hdl.handle.net/10668/19903
UL http://hdl.handle.net/10668/19903
LA en
NO Nuñez-Borque E, Betancor D, Pastor-Vargas C, Fernández-Bravo S, Martin-Blazquez A, Casado-Navarro N, et al. Personalized diagnostic approach and indirect quantification of extravasation in human anaphylaxis. Allergy. 2023 Jan;78(1):202-213
DS RISalud
RD Apr 9, 2025