RT Journal Article
T1 Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering.
A1 Ortiz-Arrabal, Olimpia
A1 Chato-Astrain, Jesus
A1 Crespo, Pascual Vicente
A1 Garzon, Ingrid
A1 Mesa-Garcia, Maria Dolores
A1 Alaminos, Miguel
A1 Gomez-Llorente, Carolina
K1 cell culture
K1 cell proliferation
K1 epithelial cells
K1 maslinic acid
K1 tissue engineering
AB In the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering.
PB Frontiers Research Foundation
SN 2296-4185
YR 2022
FD 2022-04-08
LK http://hdl.handle.net/10668/20523
UL http://hdl.handle.net/10668/20523
LA en
NO Ortiz-Arrabal O, Chato-Astrain J, Crespo PV, Garzón I, Mesa-García MD, Alaminos M, et al. Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering. Front Bioeng Biotechnol. 2022 Apr 27;10:876734.
DS RISalud
RD Apr 8, 2025