RT Journal Article
T1 Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing Olea europaea Leaf Extract.
A1 Casado-Diaz, Antonio
A1 Moreno-Rojas, Jose Manuel
A1 Verdu-Soriano, Jose
A1 Lazaro-Martinez, Jose Luis
A1 Rodriguez-Mañas, Leocadio
A1 Tunez, Isaac
A1 La Torre, Manuel
A1 Berenguer Perez, Miriam
A1 Priego-Capote, Feliciano
A1 Pereira-Caro, Gema
K1 EHO-85
K1 Olea europaea leaf extract
K1 Antioxidant activity
K1 Free radicals
AB The excess of free radicals in the wound environment contributes to its stagnation during the inflammatory phase, favoring hard-to-heal wounds. Oxidative stress negatively affects cells and the extracellular matrix, hindering the healing process. In this study, we evaluated the antioxidant and wound-healing properties of a novel multifunctional amorphous hydrogel-containing Olea europaea leaf extract (OELE). Five assessments were performed: (i) phenolic compounds characterization in OELE; (ii) absolute antioxidant activity determination in OELE and hydrogel (EHO-85); (iii) antioxidant activity measurement of OELE and (iv) its protective effect on cell viability on human dermal fibroblasts (HDFs) and keratinocytes (HaCaT); and (v) EHO-85 wound-healing-capacity analysis on diabetic mice (db/db; BKS.Cg-m+/+Leprdb). The antioxidant activity of OELE was prominent: 2220, 1558, and 1969 µmol TE/g by DPPH, ABTS, and FRAP assays, respectively. Oxidative stress induced with H2O2 in HDFs and HaCaT was normalized, and their viability increased with OELE co-treatment, thus evidencing a protective role. EHO-85 produced an early and sustained wound-healing stimulating effect superior to controls in diabetic mice. This novel amorphous hydrogel presents an important ROS scavenger capacity due to the high phenolic content of OELE, which protects skin cells from oxidative stress and contributes to the physiological process of wound healing.
PB MDPI
SN 1999-4923
YR 2022
FD 2022-01-29
LK http://hdl.handle.net/10668/21542
UL http://hdl.handle.net/10668/21542
LA en
NO Casado-Diaz A, Moreno-Rojas JM, Verdú-Soriano J, Lázaro-Martínez JL, Rodríguez-Mañas L, Tunez I, et al. Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing Olea europaea Leaf Extract. Pharmaceutics. 2022 Feb 1;14(2):349
DS RISalud
RD Apr 7, 2025