RT Journal Article
T1 First-Pass Metabolism of Chlorophylls in Mice.
A1 Viera, Isabel
A1 Chen, Kewei
A1 Ríos, José J
A1 Benito, Itziar
A1 Pérez-Gálvez, Antonio
A1 Roca, María
K1 SR-BI
K1 absorption
K1 chlorophylls
K1 liver
K1 pheophorbide
AB The dietary intake of chlorophylls is estimated to be ≈50 mg d-1 . However, their first pass metabolism and systemic assimilation is not well characterized. A group of 30 mice are fed a diet rich in chlorophylls, while 10 mice received a standard diet without chlorophylls (control group). Liver extracts are analyzed every 15 days by HPLC-ESI(+)/APCI(+)-hrTOF- MS/MS to measure the accretion of specific chlorophyll metabolites. The chlorophyll profile found in the livers of mice fed a chlorophyll-rich diet shows that the formation and/or absorption of pheophorbides, pyro-derivatives, and phytyl-chlorin e6 require the occurrence of a precise first-pass metabolism. In addition, the apical absorption of pheorphorbide a-rich micelles is significantly inhibited in Caucasian colon adenocarcinoma-2 cells pre-incubated with BLT1. Pheophorbide a absorption is, at least partly, protein-mediated through SR-BI. This active absorption process could explain the specific accumulation of pheophorbide a in the livers of animals fed a chlorophyll-rich diet. A complementary mechanism could be the de-esterification of pheophytin a in the liver, yielding pheophorbide a and phytol, which can explain the origin of phytol in the liver. Hence, the results suggest two molecular mechanisms responsible for the accumulation of the health-promoting compounds pheophorbide and phytol.
YR 2018
FD 2018-08-06
LK http://hdl.handle.net/10668/12732
UL http://hdl.handle.net/10668/12732
LA en
DS RISalud
RD Apr 8, 2025