RT Journal Article
T1 Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.
A1 Reverón-Gómez, Nazaret
A1 González-Aguilera, Cristina
A1 Stewart-Morgan, Kathleen R
A1 Petryk, Nataliya
A1 Flury, Valentin
A1 Graziano, Simona
A1 Johansen, Jens Vilstrup
A1 Jakobsen, Janus Schou
A1 Alabert, Constance
A1 Groth, Anja
K1 ChOR-seq
K1 H3K27me3
K1 H3K4me3
K1 cell cycle
K1 chromatin replication
K1 epigenetics
K1 epigenome maintenance
K1 histone modification
K1 histone recycling
K1 quantitative ChIP-seq
AB Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity.
YR 2018
FD 2018-08-23
LK https://hdl.handle.net/10668/28355
UL https://hdl.handle.net/10668/28355
LA en
DS RISalud
RD Apr 17, 2025