RT Journal Article
T1 Calprotectin protects against experimental colonic inflammation in mice.
A1 Aranda, Carlos J
A1 Ocon, Borja
A1 Arredondo-Amador, Maria
A1 Suarez, Maria Dolores
A1 Zarzuelo, Antonio
A1 Chazin, Walter J
A1 Martinez-Augustin, Olga
A1 Sanchez de Medina, Fermin
K1 Administration, Rectal
K1 Dextran Sulfate
K1 Inflammation
AB Calprotectin is a heterodimer composed of two myeloid-related proteins, S100A8 and S100A9, that is abundant in neutrophils and monocytes/macrophages. Faecal levels of calprotectin are used routinely to monitor inflammatory bowel disease activity. We aimed to assess the role of calprotectin in intestinal inflammation, using the dextran sulfate sodium model of colitis in mice. Calprotectin was administered (50 or 100 μg·day-1 ) by the intrarectal or by i.p. injection (50 μg·day-1 only). The condition of the mice was characterized by morphological and biochemical methods. Intrarectal calprotectin protected significantly against colitis, as shown by lower levels of macroscopic and microscopic damage, colonic myeloperoxidase activity and decreased expression of TNFα and toll-like receptor 4. IL-17 production by spleen and mesenteric lymph node cells was reduced. Calprotectin had no effect on body weight loss or colonic thickening. There were no effects of calprotectin after i.p. injection. Calprotectin had virtually no effects in control, non-colitic mice. Calprotectin had almost no effect on the colonic microbiota but enhanced barrier function. Treatment of rat IEC18 intestinal epithelial cells in vitro with calprotectin induced output of the chemokines CXL1 and CCL2, involving the receptor for advanced glycation end products- and NFκB. Calprotectin exerted protective effects in experimental colitis when given by the intrarectal route, by actions that appear to involve effects on the epithelium.
PB John Wiley & Sons Ltd.
YR 2018
FD 2018-03-24
LK http://hdl.handle.net/10668/12710
UL http://hdl.handle.net/10668/12710
LA en
NO Aranda CJ, Ocón B, Arredondo-Amador M, Suárez MD, Zarzuelo A, Chazin WJ, et al. Calprotectin protects against experimental colonic inflammation in mice. Br J Pharmacol. 2018 Oct;175(19):3797-3812.
NO This work was funded by the Ministerio de Economía y Competividad (SAF2011-22922, SAF2011-22812, BFU2014-57736-P and AGL2014-58883-R), Junta de Andalucía (CTS164, CTS235 and CTS6736) and the US National Institute of Allergy and Infectious Diseases (R01 AI101171). C.J.A. and B.O. are funded by the Ministry of Education, Spain. CIBERehd is funded by the Instituto de Salud Carlos III. The group is a member of the Network for Cooperative Research on Membrane Transport Proteins (REIT), co-funded by the Ministerio de Educación y Ciencia, Spain, and the European Regional Development Fund (Grant BFU2007-30688-E/BFI)
DS RISalud
RD Apr 9, 2025