%0 Journal Article
%A Garcia-Dominguez, Daniel J
%A Hontecillas-Prieto, Lourdes
%A Leon, Eduardo Andres
%A Sanchez-Molina, Sara
%A Rodriguez-Nuñez, Pablo
%A Moron, Francisco J
%A Hajji, Nabil
%A Mackintosh, Carlos
%A de-Alava, Enrique
%T An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogenesis.
%D 2020
%U http://hdl.handle.net/10668/15686
%X The presence of the chimeric EWSR1-FLI1 oncoprotein is the main and initiating event defining Ewing sarcoma (ES). The dysregulation of epigenomic and proteomic homeostasis induced by the oncoprotein contributes to a wide variety of events involved in oncogenesis and tumor progression. Attempts at studying the effects of EWSR1-FLI1 in non-tumor cells to understand the mechanisms underlying sarcomagenesis have been unsuccessful to date, as ectopic expression of EWSR1-FLI1 blocks cell cycle progression and induces apoptosis in the tested cell lines. Therefore, it is essential to find a permissive cell type for EWSR1-FLI1 expression that allows its endogenous molecular functions to be studied. Here we have demonstrated that HeLa cell lines are permissive to EWSR1-FLI1 ectopic expression, and that our model substantially recapitulates the endogenous activity of the EWSR1-FLI1 fusion protein. This model could contribute to better understanding ES sarcomagenesis by helping to understand the molecular mechanisms induced by the EWSR1-FLI1 oncoprotein.
%K Binding Sites
%K DNA
%K HeLa Cells
%K Oncogene Proteins, Fusion
%~