RT Journal Article
T1 The antioxidant effect of β-caryophyllene protects rat liver from carbon tetrachloride-induced fibrosis by inhibiting hepatic stellate cell activation.
A1 Calleja, Miguel Angel
A1 Vieites, Jose María
A1 Montero-Meterdez, Trinidad
A1 Torres, María Isabel
A1 Faus, María José
A1 Gil, Angel
A1 Suárez, Antonio
K1 Antioxidants
K1 β-Caryophyllene
K1 Liver fibrosis
K1 Hepatic stellate cells
K1 Antioxidantes
K1 Hígado
K1 Células Estrelladas Hepáticas
K1 Probucol
AB Plant-based whole foods provide thousands of bioactive metabolites to the human diet that reduce the risk of developing chronic diseases. β-Caryophyllene (CAR) is a common constituent of the essential oil of numerous plants, vegetables, fruits and medicinal herbs, and has been used as a flavouring agent since the 1930 s. Here, we report the antioxidant activity of CAR, its protective effect on liver fibrosis and its inhibitory capacity on hepatic stellate cell (HSC) activation. CAR was tested for the inhibition of lipid peroxidation and as a free radical scavenger. CAR had higher inhibitory capacity on lipid peroxidation than probucol, α-humulene and α-tocopherol. Also, CAR showed high scavenging activities against hydroxyl radical and superoxide anion. The activity of 5-lipoxygenase, an enzyme that actively participates in fibrogenesis, was significantly inhibited by CAR. Carbon tetrachloride-treated rats received CAR at 2, 20 and 200 mg/kg. CAR significantly improved liver structure, and reduced fibrosis and the expression of Col1a1, Tgfb1 and Timp1 genes. Oxidative stress was used to establish a model of HSC activation with overproduction of extracellular matrix proteins. CAR (1 and 10 μm) increased cell viability and significantly reduced the expression of fibrotic marker genes. CAR, a sesquiterpene present in numerous plants and foods, is as a natural antioxidant that reduces carbon tetrachloride-mediated liver fibrosis and inhibits hepatic cell activation.
PB Cambridge University Press
SN 0007-1145
YR 2013
FD 2013-02-14
LK http://hdl.handle.net/10668/1636
UL http://hdl.handle.net/10668/1636
LA en
NO Calleja MA, Vieites JM, Montero-Meterdez T, Torres MI, Faus MJ, Gil A, et al. The antioxidant effect of β-caryophyllene protects rat liver from carbon tetrachloride-induced fibrosis by inhibiting hepatic stellate cell activation. Br. J. Nutr. 2013,109 (3), 394-401
NO Comparative Study; Journal Article; Research Support, Non-U.S. Gov't;
DS RISalud
RD Apr 12, 2025