RT Journal Article
T1 Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines.
A1 Ripoll, Consuelo
A1 Roldan, Mar
A1 Contreras-Montoya, Rafael
A1 Diaz-Mochon, Juan J
A1 Martin, Miguel
A1 Ruedas-Rama, Maria J
A1 Orte, Angel
K1 FLIM microscopy
K1 cancer metabolism
K1 intracellular sensors
K1 nanosensing
K1 tumoral metabolism
AB The main role of mitochondria, as pivotal organelles for cellular metabolism, is the production of energy (ATP) through an oxidative phosphorylation system. During this process, the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the main features of tumoral cells is their altered metabolism, providing alternative routes to enhance proliferation and survival. Hence, it is of utmost importance to understand the relationship between mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization of different metabophenotypes.
YR 2020
FD 2020-05-25
LK http://hdl.handle.net/10668/15650
UL http://hdl.handle.net/10668/15650
LA en
DS RISalud
RD Apr 7, 2025