RT Journal Article
T1 Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions.
A1 Ulloa, Viviana
A1 Saldivia, Natalia
A1 Ferrada, Luciano
A1 Salazar, Katterine
A1 Martínez, Fernando
A1 Silva-Alvarez, Carmen
A1 Magdalena, Rocio
A1 Oviedo, María José
A1 Montecinos, Hernán
A1 Torres-Vergara, Pablo
A1 Cifuentes, Manuel
A1 Nualart, Francisco
AB Vitamin C is incorporated into the cerebrospinal fluid (CSF) through choroid plexus cells. While the transfer of vitamin C from the blood to the brain has been studied functionally, the vitamin C transporter, SVCT2, has not been detected in the basolateral membrane of choroid plexus cells. Furthermore, it is unknown how its expression is induced in the developing brain and modulated in scurvy conditions. We concluded that SVCT2 is intensely expressed in the second half of embryonic brain development and postnatal stages. In postnatal and adult brain, SVCT2 is highly expressed in all choroidal plexus epithelial cells, shown by colocalization with GLUT1 in the basolateral membranes and without MCT1 colocalization, which is expressed in the apical membrane. We confirmed that choroid plexus explant cells (in vitro) form a sealed epithelial structure, which polarized basolaterally, endogenous or overexpressed SVCT2. These results are reproduced in vivo by injecting hSVCT2wt-EYFP lentivirus into the CSF. Overexpressed SVCT2 incorporates AA (intraperitoneally injected) from the blood to the CSF. Finally, we observed in Guinea pig brain under scorbutic condition, that normal distribution of SVCT2 in choroid plexus may be regulated by peripheral concentrations of vitamin C. Additionally, we observed that SVCT2 polarization also depends on the metabolic stage of the choroid plexus cells.
YR 2019
FD 2019-10-08
LK http://hdl.handle.net/10668/14597
UL http://hdl.handle.net/10668/14597
LA en
DS RISalud
RD Apr 19, 2025