RT Journal Article
T1 Chondro-Inductive b-TPUe-Based Functionalized Scaffolds for Application in Cartilage Tissue Engineering.
A1 Martinez-Moreno, Daniel
A1 Venegas-Bustos, Desire
A1 Rus, Guillermo
A1 Galvez-Martin, Patricia
A1 Jimenez, Gema
A1 Marchal, Juan Antonio
K1 1-pyrenebutyric acid
K1 bioprinting
K1 collagen type I
K1 functionalization
K1 osteoarthritis
K1 scaffolds
AB Osteoarthritis is a disease with a great socioeconomic impact and mainly affects articular cartilage, a tissue with reduced self-healing capacity. In this work, 3D printed 1,4 butanediol thermoplastic polyurethane (b-TPUe) scaffolds are functionalized and infrapatellar mesenchymal stem cells are used as the cellular source. Since b-TPUe is a biomaterial with mechanical properties similar to cartilage, but it does not provide the desired environment for cell adhesion, scaffolds are functionalized with two methods, one based on collagen type I and the other in 1-pyrenebutiric acid (PBA) as principal components. Alamar Blue and confocal assays display that PBA functionalized scaffolds support higher cell adhesion and proliferation for the first 21 days. However, collagen type I functionalization induces higher proliferation rates and similar cell viability than the PBA method. Further, both functionalization methods induce extracellular matrix synthesis, and the presence of chondrogenic markers (Sox9, Col2a, and Acan). Finally, SEM images probe that functionalized 3D printed scaffolds present much better cell/biomaterial interactions than controls and confirm early chondrogenesis. These results indicate that the two methods of functionalization in the highly hydrophobic b-TPUe enhance the cell-biomaterial interactions and the improvement in the chondro-inductive properties, which have great potential for application in cartilage tissue engineering.
PB Wiley-VCH Verlag GmbH & Co. KGaA
YR 2022
FD 2022-07-20
LK http://hdl.handle.net/10668/19841
UL http://hdl.handle.net/10668/19841
LA en
NO Martínez-Moreno D, Venegas-Bustos D, Rus G, Gálvez-Martín P, Jiménez G, Marchal JA. Chondro-Inductive b-TPUe-Based Functionalized Scaffolds for Application in Cartilage Tissue Engineering. Adv Healthc Mater. 2022 Oct;11(19):e2200251.
DS RISalud
RD Apr 8, 2025