RT Journal Article
T1 The effects of death and post-mortem cold ischemia on human tissue transcriptomes.
A1 Ferreira, Pedro G
A1 Muñoz-Aguirre, Manuel
A1 Reverter, Ferran
A1 Sá Godinho, Caio P
A1 Sousa, Abel
A1 Amadoz, Alicia
A1 Sodaei, Reza
A1 Hidalgo, Marta R
A1 Pervouchine, Dmitri
A1 Carbonell-Caballero, Jose
A1 Nurtdinov, Ramil
A1 Breschi, Alessandra
A1 Amador, Raziel
A1 Oliveira, Patrícia
A1 Çubuk, Cankut
A1 Curado, João
A1 Aguet, François
A1 Oliveira, Carla
A1 Dopazo, Joaquin
A1 Sammeth, Michael
A1 Ardlie, Kristin G
A1 Guigó, Roderic
AB Post-mortem tissues samples are a key resource for investigating patterns of gene expression. However, the processes triggered by death and the post-mortem interval (PMI) can significantly alter physiologically normal RNA levels. We investigate the impact of PMI on gene expression using data from multiple tissues of post-mortem donors obtained from the GTEx project. We find that many genes change expression over relatively short PMIs in a tissue-specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates. By comparing ante- and post-mortem blood samples, we identify the cascade of transcriptional events triggered by death of the organism. These events do not appear to simply reflect stochastic variation resulting from mRNA degradation, but active and ongoing regulation of transcription. Finally, we develop a model to predict the time since death from the analysis of the transcriptome of a few readily accessible tissues.
YR 2018
FD 2018-02-13
LK http://hdl.handle.net/10668/12131
UL http://hdl.handle.net/10668/12131
LA en
DS RISalud
RD Apr 14, 2025