RT Journal Article
T1 An Integrative Approach of an In Vitro Measurement of the Digestibility of Triacylglycerols of Human Milk.
A1 Pérez-Gálvez, Antonio
A1 Calvo, María Visitación
A1 Aguayo-Maldonado, Josefa
A1 Fontecha, Javier
K1 bioaccessibility
K1 human colostrum
K1 in vitro digestion
K1 milk fat globule membrane
K1 milk lipids
AB Several studies have been published regarding the effect of different factors on the digestion of milk lipids, considering their natural structural arrangement as milk fat globules and the efficiency of the digestive enzymes in the lipolysis of such complex structures. During digestion, the lipolytic products are dispersed in vesicles and micelles, which are the source for absorption of digested lipids. Therefore, it is necessary to consider the isolation of the micellar phase from the digesta to appropriately determine the amounts and classes of lipids which are bioaccessible. This study presents an integrative approach that included an isolation procedure to separate the micellar fraction from undigested and non-micellar parts, and the distribution of digested milk lipids in micelles determined directly through chromatographic techniques. Four groups of five full term mothers donated colostrum or mature milk. Two sets of samples were analyzed directly (raw), and two sets were pasteurized and then analyzed. Our data revealed that the profile of digested milk lipids is different depending on the lactation period and processing stage, while the carbon atom number distribution of the digested triacylglycerols in the micellar fraction provides a substantial information regarding the acylglycerols species that are less available for absorption.
YR 2021
FD 2021-03-30
LK http://hdl.handle.net/10668/17504
UL http://hdl.handle.net/10668/17504
LA en
DS RISalud
RD Apr 19, 2025