RT Journal Article
T1 Increased 1,25(OH)2-Vitamin D Concentrations after Energy Restriction Are Associated with Changes in Skeletal Muscle Phenotype
A1 Vidal, Angela
A1 Rios, Rafael
A1 Pineda, Carmen
A1 Lopez, Ignacio
A1 Raya, Ana I.
A1 Aguilera-Tejero, Escolastico
A1 Rivero, Jose-Luis L.
K1 Energy restriction
K1 Muscle
K1 Vitamin D
K1 Rat
K1 Phenotype
K1 Kidney
K1 Diet
K1 Muscle fibers
K1 Caloric restriction
K1 Músculos
K1 Vitamina D
K1 Ratas
K1 Fenotipo
K1 Riñón
K1 Dieta
K1 Fibras musculares esqueléticas
K1 Restricción calórica
AB The influence of energy restriction (ER) on muscle is controversial, and the mechanisms are not well understood. To study the effect of ER on skeletal muscle phenotype and the influence of vitamin D, rats (n = 34) were fed a control diet or an ER diet. Muscle mass, muscle somatic index (MSI), fiber-type composition, fiber size, and metabolic activity were studied in tibialis cranialis (TC) and soleus (SOL) muscles. Plasma vitamin D metabolites and renal expression of enzymes involved in vitamin D metabolism were measured. In the ER group, muscle weight was unchanged in TC and decreased by 12% in SOL, but MSI increased in both muscles (p < 0.0001) by 55% and 36%, respectively. Histomorphometric studies showed 14% increase in the percentage of type IIA fibers and 13% reduction in type IIX fibers in TC of ER rats. Decreased size of type I fibers and reduced oxidative activity was identified in SOL of ER rats. An increase in plasma 1,25(OH)2-vitamin D (169.7 ± 6.8 vs. 85.4 ± 11.5 pg/mL, p < 0.0001) with kidney up-regulation of CYP27b1 and down-regulation of CYP24a1 was observed in ER rats. Plasma vitamin D correlated with MSI in both muscles (p < 0.001), with the percentages of type IIA and type IIX fibers in TC and with the oxidative profile in SOL. In conclusion, ER preserves skeletal muscle mass, improves contractile phenotype in phasic muscles (TC), and reduces energy expenditure in antigravity muscles (SOL). These beneficial effects are closely related to the increases in vitamin D secondary to ER.
PB MDPI
YR 2021
FD 2021-02-12
LK http://hdl.handle.net/10668/4312
UL http://hdl.handle.net/10668/4312
LA en
NO Vidal A, Rios R, Pineda C, Lopez I, Raya AI, Aguilera-Tejero E, et al. Increased 1,25(OH)2-Vitamin D Concentrations after Energy Restriction Are Associated with Changes in Skeletal Muscle Phenotype. Nutrients. 2021 Feb 12;13(2):607
DS RISalud
RD Apr 6, 2025